BRENDA - Enzyme Database
show all sequences of 3.4.21.83

Oligopeptidase B from Trypanosoma evansi. A parasite peptidase that inactivates atrial natriuretic factor in the bloodstream of infected hosts

Morty, R.E.; Pelle, R.; Vadasz, I.; Uzcanga, G.L.; Seeger, W.; Bubis, J.; J. Biol. Chem. 280, 10925-10937 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Trypanosoma evansi
Engineering
Amino acid exchange
Commentary
Organism
S563A
catalytically inactive OpdB variant, no activity with benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin and tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Inhibitors
Inhibitors
Commentary
Organism
Structure
3,4-dichloroisocoumarin
irreversible
Trypanosoma evansi
4-(2-aminoethyl)benzenesulfonyl fluoride
irreversible
Trypanosoma evansi
agmatine
reversible
Trypanosoma evansi
antipain
reversible
Trypanosoma evansi
benzamidine
reversible
Trypanosoma evansi
leupeptin
reversible
Trypanosoma evansi
phenylmethanesulfonyl fluoride
irreversible
Trypanosoma evansi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0002
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme; pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0002
-
Val-Lys-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0003
-
Val-Lys-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0006
-
Arg-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0008
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.001
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.001
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0011
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0011
-
Arg-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0011
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0012
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0012
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0013
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0013
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0016
-
Gly-Gly-Lys-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0017
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0019
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0019
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.002
-
Arg-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0021
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0021
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0021
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0022
-
Gly-Gly-Lys-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0023
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0024
-
Arg-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0027
-
Phe-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0029
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.003
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.003
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.003
-
Phe-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.003
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.003
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0033
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0038
-
Phe-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.004
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0044
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0044
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0047
-
Gly-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0048
-
Gly-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0049
-
Phe-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.005
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0102
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0122
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0135
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0143
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.04
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.048
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Atrial natriuretic factor + H2O
Trypanosoma evansi
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trypanosoma evansi
Q6QDP1
-
-
Purification (Commentary)
Commentary
Organism
-
Trypanosoma evansi
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
Trypanosoma evansi releases oligopeptidase B into the plasma of infected hosts
Trypanosoma evansi
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.017
-
-
Trypanosoma evansi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(2-furyl)acryloyl-Tyr-Lys-Arg + H2O
-
669308
Trypanosoma evansi
3-(2-furyl)acryloyl-Tyr-Lys + Arg
-
-
-
?
Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Arg-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Arg-Arg-Arg-OH + H2O
-
669308
Trypanosoma evansi
Arg-Arg + Arg
-
-
-
?
Atrial natriuretic factor + H2O
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
669308
Trypanosoma evansi
?
-
-
-
?
Atrial natriuretic factor + H2O
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor
669308
Trypanosoma evansi
?
-
-
-
?
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Gly-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Gly-Gly-Lys-Arg + H2O
-
669308
Trypanosoma evansi
Gly-Gly-Lys-Arg + Arg
-
-
-
?
Phe-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Phe-Arg-Arg + H2O
-
669308
Trypanosoma evansi
Phe-Arg + Arg
-
-
-
?
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Val-Lys-Arg-Arg + H2O
-
669308
Trypanosoma evansi
Val-Lys-Arg + Arg
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
2
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
8
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
9
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
11
-
Gly-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
12
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
12
-
Gly-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
12
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
12
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
16
-
Phe-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
17
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
17
-
Phe-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
18
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
18
-
Phe-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
18
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
21
-
Arg-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
21
-
Phe-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
22
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
23
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
26
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
29
-
Arg-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
30
-
Arg-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
33
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C,recombinant enzyme
Trypanosoma evansi
34
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
38
-
Arg-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
38
-
Val-Lys-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
40
-
Gly-Gly-Lys-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
44
-
Val-Lys-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
48
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
54
-
Gly-Gly-Lys-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
55
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C,recombinant enzyme
Trypanosoma evansi
57
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
58
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
59
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
59
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
64
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
64
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
74
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
76
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
77
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
81
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
94
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
101
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
102
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
108
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
122
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, ecombinant mutant enzyme S563A
Trypanosoma evansi
132
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
154
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10.5
pH 7.0: about 50% of maximal activity, pH 10.5: about 50% of maximal activity, hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.001
-
antipain
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.002
-
antipain
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.021
-
leupeptin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.029
-
leupeptin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.188
-
agmatine
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.207
-
agmatine
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.218
-
benzamidine
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.254
-
benzamidine
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Trypanosoma evansi
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
S563A
catalytically inactive OpdB variant, no activity with benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin and tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3,4-dichloroisocoumarin
irreversible
Trypanosoma evansi
4-(2-aminoethyl)benzenesulfonyl fluoride
irreversible
Trypanosoma evansi
agmatine
reversible
Trypanosoma evansi
antipain
reversible
Trypanosoma evansi
benzamidine
reversible
Trypanosoma evansi
leupeptin
reversible
Trypanosoma evansi
phenylmethanesulfonyl fluoride
irreversible
Trypanosoma evansi
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.001
-
antipain
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.002
-
antipain
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.021
-
leupeptin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.029
-
leupeptin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.188
-
agmatine
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.207
-
agmatine
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.218
-
benzamidine
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.254
-
benzamidine
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0002
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme; pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0002
-
Val-Lys-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0003
-
Val-Lys-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0006
-
Arg-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0008
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.001
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.001
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0011
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0011
-
Arg-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0011
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0012
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0012
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0013
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0013
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0016
-
Gly-Gly-Lys-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0017
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0019
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0019
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.002
-
Arg-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0021
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0021
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0021
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0022
-
Gly-Gly-Lys-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0023
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0024
-
Arg-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0027
-
Phe-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0029
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.003
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.003
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.003
-
Phe-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.003
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.003
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0033
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0038
-
Phe-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.004
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0044
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0044
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0047
-
Gly-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0048
-
Gly-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0049
-
Phe-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.005
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0102
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.0122
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0135
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.0143
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
0.04
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
0.048
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Atrial natriuretic factor + H2O
Trypanosoma evansi
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Trypanosoma evansi
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
Trypanosoma evansi releases oligopeptidase B into the plasma of infected hosts
Trypanosoma evansi
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.017
-
-
Trypanosoma evansi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(2-furyl)acryloyl-Tyr-Lys-Arg + H2O
-
669308
Trypanosoma evansi
3-(2-furyl)acryloyl-Tyr-Lys + Arg
-
-
-
?
Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Arg-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Arg-Arg-Arg-OH + H2O
-
669308
Trypanosoma evansi
Arg-Arg + Arg
-
-
-
?
Atrial natriuretic factor + H2O
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
669308
Trypanosoma evansi
?
-
-
-
?
Atrial natriuretic factor + H2O
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor
669308
Trypanosoma evansi
?
-
-
-
?
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Gly-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Gly-Gly-Lys-Arg + H2O
-
669308
Trypanosoma evansi
Gly-Gly-Lys-Arg + Arg
-
-
-
?
Phe-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Phe-Arg-Arg + H2O
-
669308
Trypanosoma evansi
Phe-Arg + Arg
-
-
-
?
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
Val-Lys-Arg-Arg + H2O
-
669308
Trypanosoma evansi
Val-Lys-Arg + Arg
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
2
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
8
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
9
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
11
-
Gly-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
12
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
12
-
Gly-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
12
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
12
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
16
-
Phe-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
17
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
17
-
Phe-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
18
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
18
-
Phe-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
18
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
21
-
Arg-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
21
-
Phe-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
22
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
23
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
26
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
29
-
Arg-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
30
-
Arg-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
Trypanosoma evansi
33
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C,recombinant enzyme
Trypanosoma evansi
34
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
38
-
Arg-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
38
-
Val-Lys-Arg-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
40
-
Gly-Gly-Lys-Arg
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
44
-
Val-Lys-Arg-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
48
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
54
-
Gly-Gly-Lys-Arg
pH 8.0, 37C, native enzyme
Trypanosoma evansi
55
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C,recombinant enzyme
Trypanosoma evansi
57
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
58
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
59
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
59
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
64
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
64
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
74
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
76
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
77
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
81
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
94
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
101
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
102
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
108
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
Trypanosoma evansi
122
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, ecombinant mutant enzyme S563A
Trypanosoma evansi
132
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
154
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
Trypanosoma evansi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10.5
pH 7.0: about 50% of maximal activity, pH 10.5: about 50% of maximal activity, hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Other publictions for EC 3.4.21.83
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732327
Mattiuzzo
Proteolytic activity of Escher ...
Escherichia coli
J. Microbiol. Biotechnol.
24
160-167
2014
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-
732874
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29785
Pacaud
Protease II from Escherichia c ...
Escherichia coli
J. Biol. Chem.
250
7771-7779
1975
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