Cloned (Comment) | Organism |
---|---|
Escherichia coli HB101 gene cloned and expressed in Escherichia coli JM83 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | - |
Escherichia coli | |
DFP | - |
Escherichia coli | |
leupeptin | weak | Escherichia coli | |
tosyl-Leu chloromethyl ketone | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
benzoyl-Arg 2-naphthylamide | - |
Escherichia coli | |
0.92 | - |
Benzoyl-Lys 2-naphthylamide | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
Ser532 is the reactive serine residue | Escherichia coli |
80000 | - |
1 * 80000, SDS-PAGE without mercaptoethanol | Escherichia coli |
81000 | - |
E. coli, gel filtration | Escherichia coli |
81860 | - |
E. coli, calculation from nucleotide sequence | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
HB101 gene cloned and expressed in JM83 | - |
Purification (Comment) | Organism |
---|---|
HB101 gene cloned and expressed in JM83 | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzoyl-Lys 2-naphthylamide + H2O | - |
Escherichia coli | Benzoyl-Lys + 2-naphthylamine | - |
? | |
Insulin B-chain + H2O | cleavage at the carboxyl sides of Arg and Lys residues | Escherichia coli | ? | - |
? | |
N-Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O | weak | Escherichia coli | N-Benzyloxycarbonyl-Gly + 4-nitrophenol | - |
? | |
Nalpha-benzoyl-L-Arg 2-naphthylamide + H2O | - |
Escherichia coli | Nalpha-benzoyl-L-Arg + 2-naphthylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000, SDS-PAGE without mercaptoethanol | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
30 min, pH 8.0, 50% loss of activity | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
benzoyl-Arg 2-naphthylamide | - |
Escherichia coli | |
4.4 | - |
Benzoyl-Lys 2-naphthylamide | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.2 | Escherichia coli |
8 | - |
- |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | stable | Escherichia coli |