Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropylfluorophosphate | - |
Bacillus intermedius | |
additional information | little or no inhibition with PMSF, EDTA, benzamidine, p-CMB, duck ovomucoid, inhibitor from marine anemone, and soybean trypsin inhibitor | Bacillus intermedius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilizes | Bacillus intermedius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26500 | - |
gel filtration | Bacillus intermedius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus intermedius | - |
strain 3-19 | - |
Bacillus intermedius Mrz 19 | - |
strain 3-19 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzlyoxycarbonyl-Ala-Ala-Leu-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
benzlyoxycarbonyl-Ala-Ala-Met-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
benzlyoxycarbonyl-Ala-Ala-Met-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius Mrz 19 | ? | - |
? | |
benzlyoxycarbonyl-Ala-Ala-Phe-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
benzlyoxycarbonyl-Ala-Ala-Trp-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
benzlyoxycarbonyl-Ala-Ala-Trp-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius Mrz 19 | ? | - |
? | |
benzlyoxycarbonyl-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
benzlyoxycarbonyl-Gly-Ala-Ala-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
casein + H2O | - |
Bacillus intermedius | hydrolyzed casein | - |
? | |
casein + H2O | - |
Bacillus intermedius Mrz 19 | hydrolyzed casein | - |
? | |
insulin + H2O | hydrolyzes Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 bonds in the oxidized A-chain of insulin and Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 bonds in the oxidized B-chain of insulin | Bacillus intermedius | ? | - |
? | |
insulin + H2O | hydrolyzes Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 bonds in the oxidized A-chain of insulin and Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 bonds in the oxidized B-chain of insulin | Bacillus intermedius Mrz 19 | ? | - |
? | |
additional information | does not hydrolyze the subtilisin substrate benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide, only little activity with benzyloxycarbonyl-Ala-Ala-Trp-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Leu-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Met-Asp-p-nitroanilide, and benzyloxycarbonyl-Gly-Ala-Ala-Asp-p-nitroanilide | Bacillus intermedius | ? | - |
? | |
additional information | does not hydrolyze the subtilisin substrate benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide, only little activity with benzyloxycarbonyl-Ala-Ala-Trp-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Leu-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Met-Asp-p-nitroanilide, and benzyloxycarbonyl-Gly-Ala-Ala-Asp-p-nitroanilide | Bacillus intermedius Mrz 19 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 26500, SDS-PAGE | Bacillus intermedius |
Synonyms | Comment | Organism |
---|---|---|
glutamyl endopeptidase 2 | - |
Bacillus intermedius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 37 | without Ca2+, stable at 37°C, but activity decreases at 50°C and 55°C by 55% and 90% respectively | Bacillus intermedius |
37 | 55 | with Ca2+, highly thermostable, heating at 37°C, 50°C, and 55°C has no effect on activity | Bacillus intermedius |