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Literature summary for 3.4.21.68 extracted from
- Expression of active recombinant human tissue-type plasminogen activator by using in vivo polyhydroxybutyrate granule display (2010), Appl. Environ. Microbiol., 76, 7226-7230.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a truncated version of tPA is fused to the N terminus of the phasin protein with a thrombin cleavage site as the linker, and then expressed in Escherichia coli strain XL1-Blue on the surface of polyhydroxybutyrate granules using phasin as the affinity tag. Untreate enzyme is expressed in inactive inclusion bodies in the bacteria. The in vivo surface display strategy for functional rPA expression in Escherichia coli is distinct for its efficient folding and easier purification, method optimization, overview. Subcloning in Escherichia coli strain DH5alpha | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| plasminogen + H2O | Homo sapiens | - |
plasmin + ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| plasminogen + H2O | - |
Homo sapiens | plasmin + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Tissue plasminogen activator | - |
Homo sapiens |
| tPA | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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