Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Alkalihalobacillus clausii |
Crystallization (Comment) | Organism |
---|---|
subtilisin Carlsberg covalently bound to the inhibitor L-[(1R)-1-acetamido-2-(1-naphthyl)ethyl]boronic acid, refined at 2.65 A is used in the studies | Alkalihalobacillus clausii |
the structure is determined to a resolution of 1.56 A. ISP from Bacillus clausii is dimeric, with residues from the C terminus making a major contribution to the dimer interface by crossing over to contact the partner subunit. A short N-terminal extension binds back across the active site to provide a potential novel regulatory mechanism of intrinsic proteolytic activity: a proline residue conserved throughout the ISPs introduces a kink in the polypeptide backbone that lifts the target peptide bond out of reach of the catalytic residues | Alkalihalobacillus clausii |
Protein Variants | Comment | Organism |
---|---|---|
S250A | inactive variant | Alkalihalobacillus clausii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-[(1R)-1-acetamido-2-(1-naphthyl)ethyl]boronic acid | - |
Alkalihalobacillus clausii | |
N-acetyl-L-tryptophan amide | molecular dynamics simulations are performed with subtilisin in the presence and in the absence of an inhibitor both in hexane and in water. The inhibitor induces an open conformation of the S1 pocket that is maintained after the removal of the ligand in anhydrous, but not in aqueous, simulations. The analysis of fluctuations suggest that this behavior is caused by the decreased flexibility exhibited by subtilisin in hexane | Alkalihalobacillus clausii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34780 | - |
MALDI-TOF | Alkalihalobacillus clausii |
34782 | - |
MALDI-TOF, 2 * 34782 Da | Alkalihalobacillus clausii |
55000 | - |
dimer, gel filtration | Alkalihalobacillus clausii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkalihalobacillus clausii | D0AB41 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Alkalihalobacillus clausii |
Subunits | Comment | Organism |
---|---|---|
dimer | MALDI-TOF, 2 * 34782 Da | Alkalihalobacillus clausii |
Synonyms | Comment | Organism |
---|---|---|
subtilisin | - |
Alkalihalobacillus clausii |
subtilisin A | - |
Alkalihalobacillus clausii |