Cloned (Comment) | Organism |
---|---|
full-length SUB2 gene from strain GS115 transformed into Escherichia coli. Overexpressed in Pichia pastoris KM71 | Komagataella pastoris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | totally inhibits recombinant Sub2 | Komagataella pastoris | |
Aprotinin | inhibits the enzymatic activity by 15% | Komagataella pastoris | |
chymostatin | totally inhibits recombinant Sub2 | Komagataella pastoris | |
leupeptin | inhibits the enzymatic activity by 20% | Komagataella pastoris | |
additional information | is not inhibited by 1,10-phenanthroline, EDTA, E64, bestatin, phosphoramidon and pepstatin A | Komagataella pastoris | |
phenylmethanesulfonyl fluoride | totally inhibits recombinant Sub2 | Komagataella pastoris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
sequence analysis or mass spectrometry | Komagataella pastoris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | - |
strains GS115 and KM71 | - |
Purification (Comment) | Organism |
---|---|
by ion-exchange chromatography | Komagataella pastoris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
with N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide as substrate at pH 7.0 | Komagataella pastoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Komagataella pastoris | ? | - |
? | |
additional information | Sub2 undergoes self-digestion at high concentrations | Komagataella pastoris | ? | - |
? | |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Komagataella pastoris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SUB2 | - |
Komagataella pastoris |
subtilisin | - |
Komagataella pastoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Komagataella pastoris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | - |
Komagataella pastoris |
General Information | Comment | Organism |
---|---|---|
physiological function | during growth of Pichia pastoris, Sub2 is produced as a secreted enzyme at a concentration of 10 microg/ml of culture supernatant after overexpression of the full-length SUB2 gene. During fermentative production of recombinant enzymes in methanol medium, 1 ml of Pichia pastoris culture supernatant is found to contain approximately 3 ng of Sub2, while the enzyme is not detected during growth in a medium containing glycerol as a carbon source | Komagataella pastoris |