Protein Variants | Comment | Organism |
---|---|---|
additional information | sequential randomization of 12 amino acid positions in calcium-free enzyme. Mutations increase the half-life of enzyme at elevated temperature by 15000fold and partially increase ratio of kcat/KM-value | Bacillus amyloliquefaciens |
Y217L | commercial version of enzyme, i.e. SBT* | Bacillus amyloliquefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin | mutant Y217L, pH 8.3, 25°C | Bacillus amyloliquefaciens | |
0.36 | - |
succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin | mutant Y217L, pH 8.3, 25°C | Bacillus amyloliquefaciens | |
0.45 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | mutant Y217L, pH 8.3, 25°C | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O | - |
Bacillus amyloliquefaciens | succinyl-L-Ala-L-Ala-L-Phe + 7-amino-4-methylcoumarin | - |
? | |
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O | - |
Bacillus amyloliquefaciens | succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline | - |
? | |
succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O | - |
Bacillus amyloliquefaciens | succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
subtilisin BPN | - |
Bacillus amyloliquefaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.4 | - |
succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin | mutant Y217L, pH 8.3, 25°C | Bacillus amyloliquefaciens | |
68 | - |
succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin | mutant Y217L, pH 8.3, 25°C | Bacillus amyloliquefaciens | |
210 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | mutant Y217L, pH 8.3, 25°C | Bacillus amyloliquefaciens |