Literature summary for 3.4.21.61 extracted from

Zandberg, W.F.; Benjannet, S.; Hamelin, J.; Pinto, B.M.; Seidah, N.G.
N-glycosylation controls trafficking, zymogen activation and substrate processing of proprotein convertases PC1/3 and subtilisin kexin isozyme-1 (2011), Glycobiology, 21, 1290-1300.

Search for more literature for 3.4.21.61

Cloned(Commentary)

Cloned (Comment)	Organism
expression in HEK293 cells	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
N146A	mutation results in the decreased zymogen activation of proPC1/3 and virtually inhibits its secretion	Mus musculus
N374A	mutant is processed and secreted at nearly the same rate and with the same apparent molecular mass as wild-type. Residue N374 does not appear to bear an N-glycan	Mus musculus
N618A	mutation does not significantly affect zymogen activation	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
(3S,4S,5S,6R)-2-([(2R,3S,4S,5S,6S)-3,5-dihydroxy-2-(hydroxymethyl)-6-methoxytetrahydro-2H-pyran-4-yl]amino)-6-(hydroxymethyl)tetrahydro-2H-thiopyran-3,4,5-triol	i.e. BJ-12-26-1, greatly reduces the processing of substrate proopiomelanocortin	Mus musculus
(3S,4S,5S,6R)-2-([(2S,3S,4R,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-2-methoxytetrahydro-2H-pyran-3-yl]amino)-6-(hydroxymethyl)tetrahydro-2H-thiopyran-3,4,5-triol	i.e. BJ-12-21-2, greatly reduces the processing of substrate proopiomelanocortin	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	mutation of N-glycosylation site N146 results in the decreased zymogen activation of proPC1/3 and virtually inhibits its secretion. Mutation of glycosylation site N618A mutation does not significantly affect zymogen activation. The presence, but not the exact structure, of N-glycans is crucial for proPC1/3 zymogen processing	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
proopiomelanocortin + H2O	-	Mus musculus	beta-lipotropic hormone + adrenocorticotropic hormone + ?	-	?

Synonyms

Synonyms	Comment	Organism
PC1/3	-	Mus musculus

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Release 2023.1 (January 2023)