Literature summary for 3.4.21.50 extracted from

Stressler, T.; Eisele, T.; Meyer, S.; Wangler, J.; Hug, T.; Lutz-Wahl, S.; Fischer, L.
Heterologous expression and pro-peptide supported refolding of the high specific endopeptidase Lys-C (2016), Protein Expr. Purif., 118, 31-38 .

Search for more literature for 3.4.21.50

Cloned(Commentary)

Cloned (Comment)	Organism
gene lys-C, recombinant expression gene-optimized enzyme with a short artificial N-terminal pro-peptide MGSK in Escherichia coli strains JM109(DE3) and DSM 14459 from rhamnose-inducible expression vector pJOE to 560.3 U/l activity, the pre-N-pro gene is also cloned into pJOE, resulting in expression vector pNP-2, method optimization to over 58fold increased activity, overview	Lysobacter enzymogenes

Organism

Organism	UniProt	Comment	Textmining
Lysobacter enzymogenes	-	-	-
Lysobacter enzymogenes ATCC 27796	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
denatured and solubilized recombinant Lys-C without N-terminal pro-peptide is refolded at 0.0.25 mg/ml in glutathione, N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), NDSB-201 and GuHCl buffer, 4°C, 48 h, refolding protocoll overview	Lysobacter enzymogenes

Synonyms

Synonyms	Comment	Organism
Lys-C	-	Lysobacter enzymogenes

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Lysobacter enzymogenes

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	assay at	Lysobacter enzymogenes

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Release 2023.1 (January 2023)