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Literature summary for 3.4.21.45 extracted from

  • Okroj, M.; Hsu, Y.F.; Ajona, D.; Pio, R.; Blom, A.M.
    Non-small cell lung cancer cells produce a functional set of complement factor I and its soluble cofactors (2008), Mol. Immunol., 45, 169-179.
    View publication on PubMed
Search for more literature for 3.4.21.45

Application

Application Comment Organism
medicine expression of complement factor I in lung cancer cells analyzed Homo sapiens
medicine expression of complement factor I in non-small lung cancer cells analyzed Homo sapiens
medicine expression of complement factor I in non-small lung cancer cells and complement-dependent lysis analyzed Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 light chain of Homo sapiens
50000
-
 heavy chain of Homo sapiens
88000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
complement component C3b + H2O Homo sapiens
-
 complement component C3c + ?
-
 ?
complement component C4b + H2O Homo sapiens
-
 complement component C4c + C4d
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P05156 encoded by CFI gene
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
carcinoid cell no mRNA of complement factor I detected in carcinoid cells H720, H727 Homo sapiens
-
large cell carcinoma cell line LCC, no mRNA expression detected in LCC H661 Homo sapiens
-
lung adenocarcinoma cell line AC, mRNA of complement factor I detected in H2087 and H358 but not in H1264 and A549 Homo sapiens
-
lung squamous cell carcinoma cell SCC, squamous cell carcinoma, mRNA of complement factor I detected in H226 but not in H157, H1385 Homo sapiens
-
small cell lung cancer cell SCLC, no mRNA of complement factor I detected in H69, H82, H187, H209, H345, N417, H510 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 presence of complement factor I analyzed by RT-PCR and by monitoring cleavage of complemement components C3b and C4b, assay described, protein concentrations determined by ELISA Homo sapiens
additional information
-
 presence of complement factor I analyzed by RT-PCR and by monitoring cleavage of complement components C3b and C4b, assay described, protein concentrations determined by ELISA, complement-dependent lysis of non-small cell lung cancer cells analyzed Homo sapiens
additional information
-
 presence of complement factor I analyzed by RT-PCR and by monitoring cleavage of the complement components C3b and C4b, assay described, protein concentrations determined by ELISA, complement-dependent lysis of non-small cell lung cancer cells analyzed Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C3b + H2O
-
 Homo sapiens complement component C3c + ?
-
 ?
complement component C3b + H2O production of complement factor I in non-small cell lung cancer cell lines analyzed Homo sapiens complement component C3c + ?
-
 ?
complement component C4b + H2O
-
 Homo sapiens complement component C4c + C4d
-
 ?
additional information complement factor I production analyzed by RT-PCR Homo sapiens ?
-
 ?
additional information complement factor I production analyzed, no mRNA expression detected Homo sapiens ?
-
 ?
additional information production of complement factor I analyzed, no mRNA expression detected Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 C3b and C4b cleavage assay at Homo sapiens
37
-
 cleavage assay at Homo sapiens