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Literature summary for 3.4.21.41 extracted from

  • Lacroix, M.; Ebel, C.; Kardos, J.; Dobo, J.; Gal, P.; Zavodszky, P.; Arlaud, G.J.; Thielens, N.M.
    Assembly and enzymatic properties of the catalytic domain of human complement protease C1r (2001), J. Biol. Chem., 276, 36233-36240.
    View publication on PubMed
Search for more literature for 3.4.21.41

Cloned(Commentary)

Cloned (Comment) Organism
baculovirus-mediated expression is used to produce fragments containing the SP domain and either 2 CCP modules (CCP1/2-SP) or only the second CCP module (CCP2-SP). In each case the wild-type species and two mutants stabilized in the proenzyme form by mutations at the cleavage site (R446Q) or at the active site serine residue (S637A) are produced Homo sapiens

Protein Variants

Protein Variants Comment Organism
R446Q stabilized in the single-chain proenzyme form by mutation at the cleavage site, no esterolytic activity with acetyl-Gly-Lys-methyl ester Homo sapiens
S637A stabilized in the single-chain proenzyme form by mutation at the active site serine residue, no esterolytic activity with acetyl-Gly-Lys-methyl ester Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information activated C1r and its wild-type CCP1/2-SP and CCP2-SP fragments all cleave acetyl-Gly-Lys-methyl ester with KM-values ranging from 1.3 mM to 2.1 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 molecular weight of C1r fragments Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content of C1r fragments Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Gly-Lys-methyl ester + H2O
-
 Homo sapiens ?
-
 ?
complement component C1s + H2O C1R and its activated fragments all cleave C1s, in the order of increasing efficiency: C21r, CCP1/2-SP, CCP2-SP. CCP1 is not involved in C1s recognition Homo sapiens complement component C1sbar
-
 ?

Subunits

Subunits Comment Organism
dimer CCP1 is essential to the assembly of the dimer, but formation of a stable dimer is not a prerequisite for self-activation Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information activated C1r and its wild-type CCP1/2-SP and CCP2-SP fragments all cleave acetyl-Gly-Lys-methyl ester with turnover numbers ranging from 8.2 s to 9.2 s Homo sapiens