Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzamidine | 54% inhibition at 5 mM | Mustelus mustelus | |
additional information | no or poor inhibition by pepstatin A, EDTA, 2-mercaptoethanol, and N-p-tosyl-L-phenylalanine chloromethyl ketone | Mustelus mustelus | |
N-p-tosyl-L-lysine chloromethyl ketone | 88% inhibition at 5 mM | Mustelus mustelus | |
NaCl | enzyme activity continuously decreases to 50% of maximal activity with increasing NaCl concentration of 0-30% | Mustelus mustelus | |
PMSF | 89% inhibition at 5 mM | Mustelus mustelus | |
Soybean trypsin inhibitor | 91.5% inhibition at 1 mg/ml | Mustelus mustelus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.156 | - |
Nalpha-4-tosyl-L-arginine methyl ester | pH 8.5, 25°C | Mustelus mustelus | |
0.387 | - |
N-benzoyl-DL-arginine 4-nitroanilide | pH 8.5, 25°C | Mustelus mustelus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mustelus mustelus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 65fold from intestine by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography | Mustelus mustelus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Mustelus mustelus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.65 | - |
purified enzyme, pH 8.5, 25°C | Mustelus mustelus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzoyl-DL-arginine 4-nitroanilide + H2O | - |
Mustelus mustelus | N-benzoyl-DL-arginine + 4-nitroaniline | - |
? | |
Nalpha-4-tosyl-L-arginine methyl ester + H2O | - |
Mustelus mustelus | Nalpha-4-tosyl-L-arginine + methanol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Mustelus mustelus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | profile, overview, inactivation at 70°C | Mustelus mustelus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme, 1 h, highly stable up to, unstable above | Mustelus mustelus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.62 | - |
N-benzoyl-DL-arginine 4-nitroanilide | pH 8.5, 25°C | Mustelus mustelus | |
59.15 | - |
Nalpha-4-tosyl-L-arginine methyl ester | pH 8.5, 25°C | Mustelus mustelus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Mustelus mustelus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 12 | profile, overview | Mustelus mustelus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 12 | purified enzyme, 1 h, extremely stable at pH 7.0-9.0, profile, overview | Mustelus mustelus |