Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis in a soluble active form | Streptomyces griseus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, recombinant wild-type enzyme and mutant enzyme T190P at 1.5 and 1.9 A resolution | Streptomyces griseus |
Protein Variants | Comment | Organism |
---|---|---|
T190A | the ratio of turnover number to Km-value with tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin is 47% of that of the wild-type enzyme, the ratio of turnover number to Km-value with tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin is 55% of that of the wild-type enzyme | Streptomyces griseus |
T190P | the ratio of turnover number to Km-value with tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin is 0.5% of that of the wild-type enzyme, the ratio of turnover number to Km-value with tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin is 0.7% of that of the wild-type enzyme | Streptomyces griseus |
T190S | the ratio of turnover number to Km-value with tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin is 18% of that of the wild-type enzyme, the ratio of turnover number to Km-value with tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin is 9% of that of the wild-type enzyme | Streptomyces griseus |
T190V | the ratio of turnover number to Km-value with tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin is 2.5% of that of the wild-type enzyme, the ratio of turnover number to Km-value with tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin is 0.6% of that of the wild-type enzyme | Streptomyces griseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzamidine | - |
Streptomyces griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, recombinant enzyme | Streptomyces griseus | |
0.0023 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, wild-type enzyme | Streptomyces griseus | |
0.0032 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, recombinant enzyme | Streptomyces griseus | |
0.0036 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, wild-type enzyme | Streptomyces griseus | |
0.0044 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190A | Streptomyces griseus | |
0.0061 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190S | Streptomyces griseus | |
0.0101 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190S | Streptomyces griseus | |
0.0129 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190A | Streptomyces griseus | |
0.067 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190P | Streptomyces griseus | |
0.166 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190P | Streptomyces griseus | |
0.224 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190V | Streptomyces griseus | |
0.231 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190V | Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23080 | - |
mutant enzyme T190A, electrospray mass spectrometry analysis | Streptomyces griseus |
23090 | - |
mutant enzyme T190S, electrospray mass spectrometry analysis | Streptomyces griseus |
23100 | - |
mutant enzyme T190P, electrospray mass spectrometry analysis | Streptomyces griseus |
23100 | - |
mutant enzyme T190V, electrospray mass spectrometry analysis | Streptomyces griseus |
23110 | - |
wild-type enzyme and recombinant enzyme, electrospray mass spectrometry analysis | Streptomyces griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | P00775 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces griseus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
237300 | - |
- |
Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Streptomyces griseus | ? | - |
? | |
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin + H2O | - |
Streptomyces griseus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SGT | - |
Streptomyces griseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | 1.97 | tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190P | Streptomyces griseus | |
0.5 | - |
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190V | Streptomyces griseus | |
0.52 | 0.7 | tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, wild-type enzyme | Streptomyces griseus | |
2.7 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, recombinant enzyme | Streptomyces griseus | |
3.2 | - |
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190A | Streptomyces griseus | |
8 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190P | Streptomyces griseus | |
8.78 | - |
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190P | Streptomyces griseus | |
38.33 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190V | Streptomyces griseus | |
82.5 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | pH 7.6, mutant enzyme T190A | Streptomyces griseus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
benzamidine | pH 7.6, recombinant enzyme | Streptomyces griseus | |
0.0048 | - |
benzamidine | pH 7.6, mutant enzyme T190S | Streptomyces griseus | |
0.0099 | - |
benzamidine | pH 7.6, mutant enzyme T190A | Streptomyces griseus | |
0.0164 | - |
benzamidine | pH 7.6, mutant enzyme T190P | Streptomyces griseus | |
0.197 | - |
benzamidine | pH 7.6, mutant enzyme T190V | Streptomyces griseus |