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Literature summary for 3.4.21.22 extracted from

  • Yang, L.; Manithody, C.; Qureshi, S.H.; Rezaie, A.R.
    Role of the residues of the 39-loop in determining the substrate and inhibitor specificity of factor IXa (2010), J. Biol. Chem., 285, 28488-28495.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.22

Activating Compound

Activating Compound Comment Organism Structure
Factor VIIIa
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information an FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa cleaves a FIXa-specific chromogenic substrate with normal catalytic efficiency, however, the mutant exhibits about 5fold enhanced reactivity with antothrombin specifically in the absence of the cofactor, heparin. The FIXa mutant activates factor X with about 4fold decreased kcat and about 2fold decreased Km, although the mutant interacts normally with factor VIIIa Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
antithrombin
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000038
-
 Factor X FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication Homo sapiens
0.000071
-
 Factor X wild type enzyme, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication Homo sapiens
1.2
-
 L-Leu-Gly-L-Arg-4-nitroanilide FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C Homo sapiens
2.3
-
 L-Leu-Gly-L-Arg-4-nitroanilide wild type enzyme, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Factor X + H2O Homo sapiens
-
 Factor Xa + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Factor X + H2O
-
 Homo sapiens Factor Xa + ?
-
 ?
L-Leu-Gly-L-Arg-4-nitroanilide + H2O CBS 31.39 Homo sapiens L-Leu-Gly-L-Arg + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
factor IXa
-
 Homo sapiens
FIXa
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 Factor X FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication Homo sapiens
3.5
-
 Factor X wild type enzyme, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication Homo sapiens
17.5
-
 L-Leu-Gly-L-Arg-4-nitroanilide FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C Homo sapiens
21.4
-
 L-Leu-Gly-L-Arg-4-nitroanilide wild type enzyme, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.3
-
 L-Leu-Gly-L-Arg-4-nitroanilide wild type enzyme, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C Homo sapiens
14.6
-
 L-Leu-Gly-L-Arg-4-nitroanilide FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C Homo sapiens
25000
-
 Factor X FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication Homo sapiens
49000
-
 Factor X wild type enzyme, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication Homo sapiens