Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Factor VIIIa | factor IXa acts as part of phosphocholine/phosphoserine vesicles interacting with factor VIIIa in the intrinsic tenase complex | Homo sapiens | |
heparin | activates by mediating cofactor binding, heparin binds to an exosite on the factor IXa protease domain, the factor IXa heparin-binding exosite participates in both cofactor binding and protease activation, and cofactor affinity is linked to active site conformation and factor X interaction during enzyme assembly, overview | Homo sapiens | |
Phospholipids | factor IXa acts as part of phosphocholine/phosphoserine vesicles interacting with factor VIIIa in the intrinsic tenase complex | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
stable expression of wild-type and mutant enzymes in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K126A | site-directed mutagenesis, mutation in the heparin-binding exosite, the mutant shows reduced activity and altered tenase complex formation kinetics compared to the wild-type enzyme | Homo sapiens |
K132A | site-directed mutagenesis, surface residue mutation, the mutant shows reduced activity and altered tenase complex formation kinetics compared to the wild-type enzyme | Homo sapiens |
N129A | site-directed mutagenesis, surface residue mutation, the mutant shows reduced activity and altered tenase complex formation kinetics compared to the wild-type enzyme | Homo sapiens |
N178A | site-directed mutagenesis, mutation in the heparin-binding exosite, the mutant shows reduced activity and altered tenase complex formation kinetics compared to the wild-type enzyme | Homo sapiens |
R165A | site-directed mutagenesis, mutation in the heparin-binding exosite, the mutant shows reduced activity and altered tenase complex formation kinetics compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antithrombin III | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic determination of the affinity for factor IXa-factor VIIIa complex formation, effect of ethylene glycol at 30% on reaction kinetics, overview | Homo sapiens | |
0.000001 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N178A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
0.0000052 | - |
Factor X | pH 7.4, 22°C, recombinant mutant R165A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
0.0000073 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K126A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
0.0000085 | - |
Factor X | pH 7.4, 22°C, recombinant mutants N129A and K132A, each in the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
0.0000096 | - |
Factor X | pH 7.4, 22°C, recombinant wild-type enzyme the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
0.000048 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K132A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
0.000055 | - |
Factor X | pH 7.4, 22°C, recombinant wild-type enzyme the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
0.000061 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N129A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
0.000067 | - |
Factor X | pH 7.4, 22°C, recombinant mutant R165A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
0.00008 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N178A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
0.000089 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K126A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Homo sapiens | |
Na+ | activates | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor X + H2O | Homo sapiens | the enzyme is part of the coagulation cascade, factor X activation by the intrinsic tenase complex, factor IXa-factor VIIIa, is the rate-limiting step for thrombin generation | activated factor X + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | purified factor IX is activated with human factor XIa | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | from plasma | Homo sapiens | - |
plasma | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative coagulation and amidolytic activities of recombinant wild-type and mutant enzymes, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor X + H2O | the enzyme is part of the coagulation cascade, factor X activation by the intrinsic tenase complex, factor IXa-factor VIIIa, is the rate-limiting step for thrombin generation | Homo sapiens | activated factor X + ? | - |
? | |
factor X + H2O | factor IXa acts as part of phosphocholine/phosphoserine vesicles interacting with factor VIIIa in the intrinsic tenase complex, the factor IXa heparin-binding exosite participates in both cofactor binding and protease activation, and cofactor affinity is linked to active site conformation and factor X interaction during enzyme assembly, overview | Homo sapiens | activated factor X + ? | - |
? | |
Pefachrome IXa + H2O | commercial chromogenic substrate | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
factor IXa | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
Factor X | pH 7.4, 22°C, recombinant mutant R165A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
5.08 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N178A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
5.2 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K126A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
5.47 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K132A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
5.51 | - |
Factor X | pH 7.4, 22°C, recombinant wild-type enzyme the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
5.6 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N129A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
600 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N178A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
1000 | - |
Factor X | pH 7.4, 22°C, recombinant mutant R165A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
1340 | - |
Factor X | pH 7.4, 22°C, recombinant wild-type enzyme and mutant N129A, each in the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
1780 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K132A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
1999 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K126A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |