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BRENDA support

Literature summary for 3.4.21.22 extracted from

  • Blostein, M.D.; Rigby, A.C.; Furie, B.C.; Furie, B.; Gilbert, G.E.
    Amphipathic helices support function of blood coagulation factor IXa (2000), Biochemistry, 39, 12000-12006.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Factor VIIIa 200000fold activation in the presence of phospholipid membrane through a combinantion of a decrease in Km and an increase in kcat Homo sapiens
GIGAVLKVLTTGLPALISSWIKRKRQQ approx. 600fold lower km, slight increase in kcat Homo sapiens
phospholipid membrane 200000fold activation in the presence of factor VIIIa through a combinantion of a decrease in Km and an increase in kcat Homo sapiens
TRYLRIHPQSWVHQIALRMEV approx. 600fold lower km, slight increase in kcat, optimal activation in the presence of 0.5-1 mM Ca2+ Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00013
-
Factor X 25┬░C, pH 7.4, in the presence of GIGAVLKVLTTGLPALISSWIKRKRQQ Homo sapiens
0.00017
-
Factor X 25┬░C, pH 7.4, in the presence of TRYLRIHPQSWVHQIALRMEV Homo sapiens
0.095
-
Factor X 25┬░C, pH 7.4 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, optimal concentration for cleavage of factor X by factor IXa on phospholipid membranes Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor X + H2O Homo sapiens
-
fragments of factor X
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor X + H2O
-
Homo sapiens fragments of factor X
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00016
-
Factor X 25┬░C, pH 7.4 Homo sapiens
0.00023
-
Factor X 25┬░C, pH 7.4, in the presence of GIGAVLKVLTTGLPALISSWIKRKRQQ Homo sapiens
0.00025
-
Factor X 25┬░C, pH 7.4, in the presence of TRYLRIHPQSWVHQIALRMEV Homo sapiens