Any feedback?
Literature summary for 3.4.21.21 extracted from
- Regulation of the catalytic function of coagulation factor VIIa by a conformational linkage of surface residue Glu 154 to the active site (1999), Biochemistry, 38, 2745-2751.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| tissue factor | required for hydrolysis of factor X | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant factor VII is expressed in Chinese hamster ovary cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E154A | slightly increased Km-value and decreased turnover number compared to the wild-type enzyme | Homo sapiens |
| L144A | slightly increased Km-value and decreased turnover number compared to the wild-type enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000043 | - |
Factor X | mutant enzyme L144A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles | Homo sapiens | |
| 0.000051 | - |
Factor X | mutant enzyme L192A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles | Homo sapiens | |
| 0.000059 | - |
Factor X | mutant enzyme E154A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles | Homo sapiens | |
| 0.000069 | - |
Factor X | wild-type enzyme VIIa, tissue factor/phosphatidylcholine-phosphatidylserine vesicles | Homo sapiens | |
| 0.0124 | - |
Factor X | wild-type enzyme VIIa, tissue factor/phosphatidylcholine vesicles | Homo sapiens | |
| 0.0153 | - |
Factor X | mutant enzyme K192A, tissue factor/phosphatidylcholine vesicles | Homo sapiens | |
| 0.0207 | - |
Factor X | mutant enzyme L144A, tissue factor/phosphatidylcholine vesicles | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | factor VIIa initiates the coagulation pathway upon complex formation with its cellular receptor and cofactor tissue factor | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| factor X + H2O | - |
Homo sapiens | ? | - |
? | |
| additional information | factor VIIa initiates the coagulation pathway upon complex formation with its cellular receptor and cofactor tissue factor | Homo sapiens | ? | - |
? | |
| spectrozyme Fxa + H2O | chromogenic substrate | Homo sapiens | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Factor X | mutant enzyme L144A, tissue factor/phosphatidylcholine vesicles | Homo sapiens | |
| 0.15 | - |
Factor X | mutant enzyme L192A or E154A, tissue factor/phosphatidylcholine vesicles | Homo sapiens | |
| 0.15 | - |
Factor X | mutant enzyme L144A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles | Homo sapiens | |
| 0.8 | - |
Factor X | mutant enzyme K192A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles | Homo sapiens | |
| 1.25 | - |
Factor X | mutant enzyme E154A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles | Homo sapiens | |
| 1.55 | - |
Factor X | wild-type enzyme VIIa, tissue factor/phosphatidylcholine vesicles | Homo sapiens | |
| 7.4 | - |
Factor X | wild-type enzyme VIIa, tissue factor/phosphatidylcholine-phosphatidylserine vesicles | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
