Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Tuner (DE3) cells | Tellina virus 1 |
Crystallization (Comment) | Organism |
---|---|
VP4 with the C-terminus binding into its own active site forming an intramolecular (cis) acyl-enzyme complex, hanging drop vapor diffusion method, using 21% (w/v) PEG8000 and 0.55 M ammonium sulfate | Tellina virus 1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24745 | - |
x * 24745, calculated from amino acid sequence | Tellina virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tellina virus 1 | Q2PBR5 | residue 619-830 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, SP-Sepharose column chromatography, and Sephacryl S-100 gel filtration | Tellina virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NH2-pVP2-X-VP4-VP3-COOH + H2O | VP4 is encoded as part of the 1114-residue long segment A polyprotein NH2-pVP2-X-VP4-VP3-COOH. It cleaves after amino acid residue positions 512, 618, and 830 to yield capsid precursor protein pVP2, peptide X, VP4 itself, and ribonucleoprotein VP3. Additional cleavage sites were found at the C-terminal side of amino acids 451, 492, and 499 within the pVP2 region | Tellina virus 1 | pVP2 + peptide X + VP4 + VP3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24745, calculated from amino acid sequence | Tellina virus 1 |
Synonyms | Comment | Organism |
---|---|---|
VP4 | - |
Tellina virus 1 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Tellina virus 1 | calculated from amino acid sequence | - |
9.9 |