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Literature summary for 3.4.21.111 extracted from

  • Kurosaka, K.; Ohta, T.; Matsuzawa, H.
    A 38 kDa precursor protein of aqualysin I (a thermophilic subtilisin-type protease) with a C-terminal extended sequence: its purification and in vitro processing (1996), Mol. Microbiol., 20, 385-389.
    View publication on PubMed
Search for more literature for 3.4.21.111

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus aquaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide 40°C, pH 7.5, precursor protein Thermus aquaticus
0.05
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide 40°C, pH 7.5, mature protein Thermus aquaticus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane precursor protein Thermus aquaticus 16020
-
soluble mature protein Thermus aquaticus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 mature protein after heat treatment, SDS-PAGE Thermus aquaticus
38000
-
 precursor protein, SDS-PAGE Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification intramolecular self-processing mechanism Thermus aquaticus

Purification (Commentary)

Purification (Comment) Organism
38000 Da precursor protein Thermus aquaticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Thermus aquaticus ?
-
 ?
N-succinyl-Phe-Nle-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Nle-Ala + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Thermus aquaticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20400
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide 40°C, pH 7.5, precursor protein Thermus aquaticus
47400
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide 40°C, pH 7.5, mature protein Thermus aquaticus