Cloned (Comment) | Organism |
---|---|
expression of His-tagged amino acids 596-855 of matriptase in Escherichia coli strain M15 | Homo sapiens |
into pQE-vecto and expressed in Escherichia coli M15 strain | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | wild type eglin c has no effect | Homo sapiens | |
R1K'4-eglin | different eglin c variants with differing inhibitory potential versus matriptase, construction, expression and purification of eglin c variants and screening for inhibitory potency, overview, R1K'4-eglin has the wild-type Pro45 at P1 position and Tyr49 at P4' position residues replaced with Arg and Lys, respectively, leads to the production of a selective, high affinity and proteolytically stable inhibitor of matriptase, molecular modeling of enzyme-inhibitor complex, overview | Homo sapiens | |
R1K4-eglin | wild type eglin c with Pro45 at P1 position and Tyr49 residues at P4 position replaced with Arg and Lys respectively, most potent, selective, high affinity and proteolytically stable inhibitor | Homo sapiens | |
R4R1-eglin | with substituted P42 and L45 for arginine residues, variants containing an Arg or Lys at position 49 instead of the original Tyr residue show enhanced inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | matriptase is a type II transmembrane serine protease | Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
refolded zymogen | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by immobilized metal-chelate affinity chromatography and ion exchange chromatography | Homo sapiens |
recombinant His-tagged amino acids 596-855 of matriptase from Escherichia coli strain M15 by metal affinity chromatography, dialysis, and anion exchange chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epithelial cancer cell | overexpression of the enzyme | Homo sapiens | - |
epithelium | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
Boc-Glu-Ala-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Boc-Glu-Ala-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs ot the type II transmembrane serine protease family | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Homo sapiens |
9 | 11 | purified enzyme | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000004 | - |
R1K4-eglin | - |
Homo sapiens | |
0.000004 | - |
R1K'4-eglin | pH 9.0, recombinant His-tagged amino acids 596-855 of matriptase | Homo sapiens | |
0.000026 | - |
R4R1-eglin | - |
Homo sapiens |