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Literature summary for 3.4.21.108 extracted from

  • Khan, F.S.; Fujioka, M.; Datta, P.; Fernandes-Alnemri, T.; Jaynes, J.B.; Alnemri, E.S.
    The interaction of DIAP1 with dOmi/HtrA2 regulates cell death in Drosophila (2008), Cell Death Differ., 15, 1073-1083.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.108

Cloned(Commentary)

Cloned (Comment) Organism
constructs encoding C-terminal His6-tagged full-length dOmi or truncated mutants are generated using the pET-21a and the pVL-1393 transfer vector, proteins are overexpressed in Escherichia coli BL21DE3 and Sf9 insect cells, respectively Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol released into the cytosol during apoptosis Drosophila melanogaster 5829
-
mitochondrial intermembrane space
-
 Drosophila melanogaster 5758
-
mitochondrion
-
 Drosophila melanogaster 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 dOmi-S, determined by SDS-PAGE and Western Blot analysis Drosophila melanogaster
35000
-
 dOmi-L, determined by SDS-PAGE and Western Blot analysis Drosophila melanogaster
38000
-
 unprocessed dOmi, determined by SDS-PAGE and Western Blot analysis Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Drosophila melanogaster cleavage of DIAP1 between the BIR1 and BIR2 domain and further degradation of BIR1 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9VFJ3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Drosophila melanogaster
ubiquitination
-
 Drosophila melanogaster

Purification (Commentary)

Purification (Comment) Organism
using TALON agarose Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
eye eye disc Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cleavage of DIAP1 between the BIR1 and BIR2 domain and further degradation of BIR1 Drosophila melanogaster ?
-
 ?

Synonyms

Synonyms Comment Organism
dOmi/HtrA2 two isoforms, dOmi-L, dOmi-S Drosophila melanogaster
Omi/HtrA2
-
 Drosophila melanogaster
Omi/HtrA2 serine protease
-
 Drosophila melanogaster