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Literature summary for 3.4.21.107 extracted from
- Purification, biochemical characterization and structural modeling of a potential htrA-like serine protease from Bacillus subtilis DR8806 (2015), J. Mol. Catal. B, 115, 51-58.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| modeling of 3D structure | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 1 mM, complete inhibition | Bacillus subtilis |  |
| PMSF | 5 mM, 60% residual activity, 10 mM, no residual activity | Bacillus subtilis | |
| Sodium dodecyl sulfate | 1 mM, 61% resiudal activity | Bacillus subtilis | |
| Triton X-100 | 5 mM, 71% residual activity | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, up to 129% stimulation of the protease activity | Bacillus subtilis | |
| Fe2+ | 10 mM, up to 112% stimulation of the protease activity | Bacillus subtilis | |
| K+ | 10 mM, up to 134% stimulation of the protease activity | Bacillus subtilis | |
| Mg2+ | 10 mM, up to 128% stimulation of the protease activity | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis DR8806 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 79.3 | - |
pH 8.0, 37°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Bovine serum albumin + H2O | 85% of the activity with casein | Bacillus subtilis | ? | - |
? | |
| Bovine serum albumin + H2O | 85% of the activity with casein | Bacillus subtilis DR8806 | ? | - |
? | |
| casein + H2O | - |
Bacillus subtilis | ? | - |
? | |
| casein + H2O | - |
Bacillus subtilis DR8806 | ? | - |
? | |
| Gelatin + H2O | 20% of the activity with casein | Bacillus subtilis | ? | - |
? | |
| Gelatin + H2O | 20% of the activity with casein | Bacillus subtilis DR8806 | ? | - |
? | |
| N-acetyl-L-tyrosine ethyl ester + H2O | 51% of the activity with casein | Bacillus subtilis | N-acetyl-L-tyrosine + ethanol | - |
? | |
| N-acetyl-L-tyrosine ethyl ester + H2O | 51% of the activity with casein | Bacillus subtilis DR8806 | N-acetyl-L-tyrosine + ethanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 85 | at least 50% of maximum activity | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, 70% residual activity | Bacillus subtilis |
| 70 | - |
1 h, 30% residual activity | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | - |
Bacillus subtilis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus subtilis | isoeletric focusing | - |
6.6 |
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