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Literature summary for 3.4.21.107 extracted from

  • Hadfield, K.D.; Rock, C.F.; Inkson, C.A.; Dallas, S.L.; Sudre, L.; Wallis, G.A.; Boot-Handford, R.P.; Canfield, A.E.
    HtrA1 inhibits mineral deposition by osteoblasts. Requirement for the protease and PDZ domains (2008), J. Biol. Chem., 283, 5928-5938.
    View publication on PubMed
Search for more literature for 3.4.21.107

Cloned(Commentary)

Cloned (Comment) Organism
cloning from MC3T3-E1 cells and expression in avian myeloblastosis virus reverse transcriptase and oligo(dT) primers, overexpression in 2T3 osteoblasts and secretion of the recombinant enzyme to the cell culture medium, expression of HtrA1 in 293-EBNA cells using a mammalian episomal expression vector pCEP-His and secretion of recombinant enzyme to the culture medium Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information downregulation of HtrA1 by siRNA increases osteoblast mineralization, HtrA1 overexpression inhibits osteoblast mineral deposition in vitro and prevents BMP-2-induced mineralization, overview Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
decorin + H2O Mus musculus the small leucine-rich proteoglycan is highly expressed in bone and regulates type I collagen fibril assembly decorin peptide fragments generation of fragments ranging from 150 to 75 kDa ?
fibronectin + H2O Mus musculus a major noncollagenous component of mineralized bone matrix fibronectin peptide fragments generation of fragments ranging from 200 to 150 kDa ?
matrix Gla protein + H2O Mus musculus the protein substrate is present in cartilage, bone, and arteries processed matrix Gla protein + 12 kDa peptide
-
 ?
additional information Mus musculus HtrA1 degrades specific matrix-associated proteins, HtrA1 inhibits mineral deposition by osteoblasts, the protease domain and the PDZ domain are essential for the inhibition of osteoblast mineralization by HtrA1, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant HtrA1 from 293-EBNA cell culture medium partially by ultrafiltration and dialysis Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
MC3T3-E1 cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-casein + H2O
-
 Mus musculus beta-casein peptide fragments
-
 ?
decorin + H2O the small leucine-rich proteoglycan is highly expressed in bone and regulates type I collagen fibril assembly Mus musculus decorin peptide fragments generation of fragments ranging from 150 to 75 kDa ?
decorin + H2O a small leucine-rich proteoglycan, human substrate Mus musculus decorin peptide fragments generation of fragments ranging from 150 to 75 kDa ?
fibronectin + H2O a major noncollagenous component of mineralized bone matrix Mus musculus fibronectin peptide fragments generation of fragments ranging from 200 to 150 kDa ?
fibronectin + H2O human plasma-derived substrate Mus musculus fibronectin peptide fragments generation of fragments ranging from 200 to 150 kDa ?
matrix Gla protein + H2O the protein substrate is present in cartilage, bone, and arteries Mus musculus processed matrix Gla protein + 12 kDa peptide
-
 ?
matrix Gla protein + H2O cleavage of MGP at the C terminus Mus musculus processed matrix Gla protein + 12 kDa peptide
-
 ?
additional information HtrA1 degrades specific matrix-associated proteins, HtrA1 inhibits mineral deposition by osteoblasts, the protease domain and the PDZ domain are essential for the inhibition of osteoblast mineralization by HtrA1, overview Mus musculus ?
-
 ?
additional information HtrA1 is a secreted multidomain protein with serine protease activity Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
HtrA1
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Mus musculus