Cloned (Comment) | Organism |
---|---|
cloned into a Bluescript plasmid and overexpressed in Escherichia coli DH1, disrupted gene transformed to Escherichia coli Jc7623 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption of the ptd gende to obtain lack of activity of protease Do, mutant with prolonged lag period, reduced ability to degrade cell proteins and unable to survive at high temperatures | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strains DH1 and JC7623 (recBc, sbcB) | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
HtrA | - |
Escherichia coli |
protease do | - |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | no change of cell density of wild type, cell density of the mutant rapidly falls, protease Do essential for the cell's survival at high temperatures | Escherichia coli |