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Literature summary for 3.4.21.104 extracted from

  • Matsushita, M.; Fujita, T.
    Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-lik serine protease (1992), J. Exp. Med., 176, 1497-1502.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.104

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 1 * 66000, heavy subunit, + 1 * 31000, light subunit, SDS-PAGE Homo sapiens
66000
-
 1 * 66000, heavy subunit, + 1 * 31000, light subunit, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
complement component C2 + H2O Homo sapiens
-
 2 fragments of complement component C2
-
 ?
complement component C4 + H2O Homo sapiens
-
 2 fragments of complement C4
-
 ?
additional information Homo sapiens enzyme is Mg2+-dependently associated with the mannose-binding protein, a C-type lectin binding to terminal mannose and N-acetylglucosamine moieties on surfaces of certain pathogens and activating the classical complement pathway, the enzyme is not identical with C1r or C1s ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from serum by sequential affinity chromatography, separation from serum mannose-binding protein MBP in presence of EDTA Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
Selective cleavage after Arg223 in complement component C2 (-Ser-Leu-Gly-Arg-/-Lys-Ile-Gln-Ile) and after Arg76 in complement component C4 (-Gly-Leu-Gln-Arg-/-Ala-Leu-Glu-Ile) catalytic serine residue is located on the light chain subunit Homo sapiens
a

Renatured (Commentary)

Renatured (Comment) Organism
combination of isolated MASP and MBP restores the complex and the complement activating activity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C2 + H2O
-
 Homo sapiens 2 fragments of complement component C2
-
 ?
complement component C4 + H2O
-
 Homo sapiens 2 fragments of complement C4
-
 ?
complement component C4 + H2O
-
 Homo sapiens 2 fragments of complement C4 a fragment corresponding to C4b with appearance of the alpha'-chain ?
additional information enzyme is Mg2+-dependently associated with the mannose-binding protein, a C-type lectin binding to terminal mannose and N-acetylglucosamine moieties on surfaces of certain pathogens and activating the classical complement pathway, the enzyme is not identical with C1r or C1s Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 66000, heavy subunit, + 1 * 31000, light subunit, SDS-PAGE Homo sapiens
More the subunits are linked by disulfide bonds Homo sapiens

Synonyms

Synonyms Comment Organism
MASP
-
 Homo sapiens
MBP-associated serine protease
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens