Crystallization (Comment) | Organism |
---|---|
wild-type and mutants C108A and H218N in complex with substrate methotrexate pentaglutamate and product methotrexate glutamate, to 1.9 to 2.4 A resolution. The side chain of residue Phe20 and the 6-methylpterin ring of methotrexate pentaglutamate invoke pi-pi interactions to promote distinct concerted conformational alterations involving about 90° rotations in the complexes with the C108A and H218N mutant proteins | Danio rerio |
Protein Variants | Comment | Organism |
---|---|---|
C108A | complete loss of hydrolytic activity | Danio rerio |
F20A | about 40% decrease in hyrolytic activity | Danio rerio |
F20A/C108A | complete loss of hydrolytic activity | Danio rerio |
F20R | about 40% increase in hyrolytic activity | Danio rerio |
F20R/C108A | complete loss of hydrolytic activity | Danio rerio |
H218N | complete loss of hydrolytic activity. Residue His218 alone suffices to catalyze the hydrolysis of the gamma-glutamate bond in gamma-glutamyl hydrolase | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q6NY42 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methotrexate pentaglutamate + H2O | - |
Danio rerio | methotrexate glutamate + tetra-gamma-L-glutamate | - |
? |