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Literature summary for 3.4.19.13 extracted from
- gamma-Glutamyl transpeptidase architecture: Effect of extra sequence deletion on autoprocessing, structure and stability of the protein from Bacillus licheniformis (2014), Biochim. Biophys. Acta, 1844, 2290-2297.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTAY385-E398 | no autocatalytic processing, no catalytic activity | Bacillus licheniformis |
| DELTAY385-I387 | mutant is able to autoprocess itself, leading to the formation of a small and a large subunit with masses of about 41 kDa and 22 kDa, respectively. Mutant shows increase in catalytic efficiency | Bacillus licheniformis |
| DELTAY385-I396 | mutant is able to autoprocess itself, albeit slowly, leading to the formation of a small and a large subunit with masses of about 41 kDa and 22 kDa, respectively. Mutant shows decrease in catalytic efficiency | Bacillus licheniformis |
| DELTAY385-K394 | mutant is able to autoprocess itself, albeit slowly, leading to the formation of a small and a large subunit with masses of about 41 kDa and 22 kDa, respectively. Mutant shows decrease in catalytic efficiency | Bacillus licheniformis |
| DELTAY385-P391 | mutant is able to autoprocess itself, albeit slowly, leading to the formation of a small and a large subunit with masses of about 41 kDa and 22 kDa, respectively. Mutant shows decrease in catalytic efficiency | Bacillus licheniformis |
| DELTAY385-V388 | mutant is able to autoprocess itself, leading to the formation of a small and a large subunit with masses of about 41 kDa and 22 kDa, respectively. Mutant shows increase in catalytic efficiency | Bacillus licheniformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.37 | - |
L-glutamic acid-(4-nitroanilide) | wild-type, pH 8.0, 25°C | Bacillus licheniformis |  |
| 0.57 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-V388, pH 8.0, 25°C | Bacillus licheniformis | |
| 0.74 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-K394, pH 8.0, 25°C | Bacillus licheniformis | |
| 0.76 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-P391, pH 8.0, 25°C | Bacillus licheniformis | |
| 0.81 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-I387, pH 8.0, 25°C | Bacillus licheniformis | |
| 1.06 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-I396, pH 8.0, 25°C | Bacillus licheniformis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | A9YTT0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamic acid-(4-nitroanilide) + H2O | - |
Bacillus licheniformis | 4-nitroaniline + L-glutamate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45.3 | 47.9 | melting temoeratures of deletion mutants | Bacillus licheniformis |
| 60 | - |
or above, melting temerature of wild-type | Bacillus licheniformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.09 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-I396, pH 8.0, 25°C | Bacillus licheniformis | |
| 3.01 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-K394, pH 8.0, 25°C | Bacillus licheniformis | |
| 4.74 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-P391, pH 8.0, 25°C | Bacillus licheniformis | |
| 12.45 | - |
L-glutamic acid-(4-nitroanilide) | wild-type, pH 8.0, 25°C | Bacillus licheniformis | |
| 24.41 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-V388, pH 8.0, 25°C | Bacillus licheniformis | |
| 55.11 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-I387, pH 8.0, 25°C | Bacillus licheniformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
wild-type as well as deletion mutants | Bacillus licheniformis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.97 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-I396, pH 8.0, 25°C | Bacillus licheniformis | |
| 4.09 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-K394, pH 8.0, 25°C | Bacillus licheniformis | |
| 6.2 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-P391, pH 8.0, 25°C | Bacillus licheniformis | |
| 27.22 | - |
L-glutamic acid-(4-nitroanilide) | wild-type, pH 8.0, 25°C | Bacillus licheniformis | |
| 43.03 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-V388, pH 8.0, 25°C | Bacillus licheniformis | |
| 67.92 | - |
L-glutamic acid-(4-nitroanilide) | mutant DELTAY385-I387, pH 8.0, 25°C | Bacillus licheniformis | |
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