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Literature summary for 3.4.19.13 extracted from

  • Kushwaha, N.; Srivastava, S.
    Gamma-glutamyl transpeptidase from two plant growth promoting rhizosphere fluorescent pseudomonads (2014), Antonie van Leeuwenhoek, 105, 45-56.
    View publication on PubMed
Search for more literature for 3.4.19.13

Inhibitors

Inhibitors Comment Organism Structure
Na+ 0.2 M, 80% residual activity Pseudomonas fluorescens

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Pseudomonas fluorescens
-
-
periplasm
-
 Pseudomonas protegens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 50 mM, 320% of initial activity Pseudomonas fluorescens
Co2+ 1 mM, 217% of initial activity Pseudomonas fluorescens
Mg2+ 50 mM, 313% of initial activity Pseudomonas fluorescens
Mn2+ 50 mM, 301% of initial activity Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 and 40000, SDS-PAGE, small and large subunit Pseudomonas fluorescens
40000
-
 and 23000, SDS-PAGE, large and small subunit Pseudomonas fluorescens
57330
-
 unprocessed protein, calculated Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens Q4KIM5 enzyme displays both glutathione hydrolase and gamma-glutamyl transpeptidase activity, EC 2.3.2.2
-
Pseudomonas fluorescens Pf-5 Q4KIM5 enzyme displays both glutathione hydrolase and gamma-glutamyl transpeptidase activity, EC 2.3.2.2
-
Pseudomonas protegens L7Z147 enzyme displays both glutathione hydrolase and gamma-glutamyl transpeptidase activity, EC 2.3.2.2
-
Pseudomonas protegens PfT-1 L7Z147 enzyme displays both glutathione hydrolase and gamma-glutamyl transpeptidase activity, EC 2.3.2.2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification protein is processed to subunits of 23000 and 40000 Da Pseudomonas fluorescens
proteolytic modification protein is processed to subunits of 23000 and 40000 Da Pseudomonas protegens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31.06
-
 pH 8.0, 50°C Pseudomonas fluorescens
31.06
-
 pH 8.0, 50°C Pseudomonas protegens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamic acid 4-nitroanilide + H2O
-
 Pseudomonas fluorescens 4-nitroaniline + L-glutamate
-
 ?
L-glutamic acid 4-nitroanilide + H2O
-
 Pseudomonas fluorescens Pf-5 4-nitroaniline + L-glutamate
-
 ?
L-glutamic acid-(4-nitroanilide) + H2O
-
 Pseudomonas protegens 4-nitroaniline + L-glutamate
-
 ?
L-glutamic acid-(4-nitroanilide) + H2O
-
 Pseudomonas protegens PfT-1 4-nitroaniline + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 23000 + 1 * 40000, SDS-PAGE Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas fluorescens
50
-
-
 Pseudomonas protegens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
 Pseudomonas fluorescens
30 60
-
 Pseudomonas protegens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 24 h, 50% residual activity Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas fluorescens
8
-
-
 Pseudomonas protegens

pH Range

pH Minimum pH Maximum Comment Organism
4 12
-
 Pseudomonas fluorescens
4 12
-
 Pseudomonas protegens