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Literature summary for 3.4.19.1 extracted from
- Molecular cloning, expression and characterization of acylpeptide hydrolase in the silkworm, Bombyx mori (2016), Gene, 580, 8-16 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene APH, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21 Transetta (DE3) | Bombyx mori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chlorpyrifos | significantly inhibits the activity of enzyme APH both in vitro and in vivo | Bombyx mori |  |
| malathion | significantly inhibits the activity of enzyme APH both in vitro and in vivo | Bombyx mori | |
| phoxim | significantly inhibits the activity of enzyme APH both in vitro and in vivo | Bombyx mori | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.82 | - |
N-acetyl-L-Ala-4-nitroanilide | pH 7.5, 25°C, recombinant enzyme | Bombyx mori | |
| 0.83 | - |
N-acetyl-L-Ala-4-nitroanilide | pH 7.5, 45°C, recombinant enzyme | Bombyx mori | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| basement membrane | - |
Bombyx mori | 5604 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | A0A0X9PFK4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 Transetta (DE3) by nickel affinity chromatography | Bombyx mori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| midgut | - |
Bombyx mori | - |
| additional information | enzyme BmAPH is expressed in all tested tissues and developmental stages of the silkworm, immunohistochemistry analysis. BmAPH protein is localized in the basement membranes | Bombyx mori | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-L-Ala-4-nitroanilide + H2O | - |
Bombyx mori | N-acetyl-L-Ala + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78500, about, sequence calculation, x * 80000, recombinant His-tagged enzyme, SDS-PAGE | Bombyx mori |
| More | enzyme peptide mapping, mass spectrometric analysis, overview | Bombyx mori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acylpeptide hydrolase | - |
Bombyx mori |
| APH | - |
Bombyx mori |
| BmAPH | - |
Bombyx mori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant enzyme | Bombyx mori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant enzyme | Bombyx mori |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| additional information | - |
the IC50 values of chlorpyrifos, phoxim, and malathion with recombinant enzyme are 1.60, 7.90, and 7.62 mg/l, respectively, pH 7.5, 25°C | Bombyx mori | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the catalytic triad is composed by catalytic residues Ser566, Asp654, and His686 | Bombyx mori |
| physiological function | enzyme BmAPHmay be involved in enhancing silkworm tolerance to organophosphorus (OP) insecticides | Bombyx mori |
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