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Literature summary for 3.4.18.1 extracted from

  • Bak, H.J.; Kim, M.S.; Kim, N.Y.; Go, H.J.; Han, J.W.; In Jo, H.; Ahn, S.J.; Park, N.G.; Chung, J.K.; Lee, H.H.
    Molecular cloning, expression, and enzymatic analysis of cathepsin X from starfish (Asterina pectinifera) (2013), Appl. Biochem. Biotechnol., 169, 847-861.
    View publication on PubMed
Search for more literature for 3.4.18.1

Activating Compound

Activating Compound Comment Organism Structure
Brij 35 0.01%, 125% of initial activity Patiria pectinifera
KCl 1 mM, 116% of initial activity Patiria pectinifera
Triton X-100 0.01%, 123% of initial activity Patiria pectinifera
Tween 20 0.01%, 130% of initial activity Patiria pectinifera

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Patiria pectinifera

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.1 mM, 89% inhibition Patiria pectinifera
antipain 0.1 mM, 62% inhibition Patiria pectinifera
Co2+ 1 mM, 71% inhibition Patiria pectinifera
Cu2+ 1 mM, 93% inhibition Patiria pectinifera
EDTA 0.1 mM, 86% inhibition Patiria pectinifera
EGTA 0.1 mM, 84% inhibition Patiria pectinifera
Hg2+ 1 mM, 90% inhibition Patiria pectinifera
N-ethylmaleimide 0.1 mM, 86% inhibition Patiria pectinifera
sodium dodecylsulfate 0.01%, 15.6% residual activity Patiria pectinifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32700
-
 x * 32700, calculated Patiria pectinifera

Organism

Organism UniProt Comment Textmining
Patiria pectinifera G1JRK2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification
-
 Patiria pectinifera

Source Tissue

Source Tissue Comment Organism Textmining
liver most abundant expression Patiria pectinifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 61% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O 34% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O 54% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera benzyloxycarbonyl-Leu-Leu-Glu + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Patiria pectinifera benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O 12% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 32700, calculated Patiria pectinifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Patiria pectinifera

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Patiria pectinifera