KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
folate | - |
Pseudomonas sp. | |
0.008 | - |
methotrexate | - |
Pseudomonas sp. | |
0.034 | - |
5-methyltetrahydrofolate | - |
Pseudomonas sp. | |
0.12 | - |
5-formyltetrahydrofolate | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains 4 atoms of zinc per enzyme molecule, which are required for full activity | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41800 | - |
2 * 41800, SDS-PAGE | Pseudomonas sp. |
83000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
large scale | Pseudomonas sp. |
strain RS-16 | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
215 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formyltetrahydrofolic acid + H2O | - |
Pseudomonas sp. | 5-formylpteroate + Glu | - |
? | |
5-methyltetrahydrofolate + H2O | - |
Pseudomonas sp. | 5-methylpteroate + Glu | - |
? | |
folic acid + H2O | - |
Pseudomonas sp. | pteroic acid + glutamic acid | - |
? | |
methotrexate + H2O | - |
Pseudomonas sp. | 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41800, SDS-PAGE | Pseudomonas sp. |