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Literature summary for 3.4.17.10 extracted from

  • Fricker, L.D.
    Carboxypeptidase E (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ) Academic Press, 2, 840-844.
No PubMed abstract available
Search for more literature for 3.4.17.10

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, localization on chromosome 4, several naturally occurring genetic variants exist Homo sapiens
DNA and amino acid sequence determination, localization on chromosome 8 Mus musculus
high level expression in insect cells using the baculovirus transfection system, the recombinant enzyme is indistinguishable from the native enzyme, expression in bacteria results in inactive enzyme Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
R283W naturally occurring genetic variant of the enzyme, the mutant shows altered kinetics, heat stability, and pH optimum, and a 50% reduced activity compared to the wild-type enzyme Homo sapiens
S202P enzyme-defective mutant CPEfat/fat mice show a point mutation in the coding region of the gene encoding the enzyme, and lack enzyme activity completely, which affects peptide processing the mutant mice Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Aplysia sp.
1,10-phenanthroline
-
 Bos taurus
1,10-phenanthroline
-
 Homo sapiens
1,10-phenanthroline
-
 Mus musculus
1,10-phenanthroline
-
 Rattus norvegicus
4-Chloromercuriphenylsulfonate over 95% inhibition at 0.1 mM Bos taurus
HgCl2
-
 Aplysia sp.
HgCl2 over 95% inhibition at 0.01 mM Bos taurus
HgCl2
-
 Homo sapiens
HgCl2
-
 Mus musculus
HgCl2
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion from neuroendocrine tissue Mus musculus
-
-
extracellular secretion from neuroendocrine tissue Homo sapiens
-
-
extracellular secretion from neuroendocrine tissue Rattus norvegicus
-
-
extracellular secretion from neuroendocrine tissue Bos taurus
-
-
extracellular secretion from neuroendocrine tissue Aplysia sp.
-
-
membrane
-
 Bos taurus 16020
-
membrane the enzyme is sorted into the secretory pathway via its C-terminal domain Mus musculus 16020
-
membrane the enzyme is sorted into the secretory pathway via its C-terminal domain Homo sapiens 16020
-
membrane the enzyme is sorted into the secretory pathway via its C-terminal domain Rattus norvegicus 16020
-
membrane the enzyme is sorted into the secretory pathway via its C-terminal domain Aplysia sp. 16020
-
vesicle enkephalin-containing chromaffin vesicles in the adrenal medulla, the enzyme is sorted into the secretory pathway via its C-terminal domain Bos taurus 31982
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates in the millimolar concentration range Mus musculus
Co2+ stimulates in the millimolar concentration range Homo sapiens
Co2+ stimulates in the millimolar concentration range Rattus norvegicus
Co2+ stimulates in the millimolar concentration range Bos taurus
Co2+ stimulates in the millimolar concentration range Aplysia sp.
additional information metallocarboxypeptidase Mus musculus
additional information metallocarboxypeptidase Homo sapiens
additional information metallocarboxypeptidase Rattus norvegicus
additional information metallocarboxypeptidase Bos taurus
additional information metallocarboxypeptidase Aplysia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aplysia sp. the enzyme contributes to the sorting of proteins into the secretory pathway ?
-
 ?
additional information Mus musculus the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters ?
-
 ?
additional information Homo sapiens the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters ?
-
 ?
additional information Rattus norvegicus the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters ?
-
 ?
additional information Bos taurus the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aplysia sp.
-
-
-
Bos taurus
-
-
-
Homo sapiens
-
 different genetic variants
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glycosylation does not appear to affect the enzyme activity Mus musculus
glycoprotein glycosylation does not appear to affect the enzyme activity Homo sapiens
glycoprotein glycosylation does not appear to affect the enzyme activity Rattus norvegicus
glycoprotein glycosylation does not appear to affect the enzyme activity Bos taurus
glycoprotein glycosylation does not appear to affect the enzyme activity Aplysia sp.

Purification (Commentary)

Purification (Comment) Organism
native enzyme from brain to homogeneity by 4-aminobenzoyl affinity chromatography Rattus norvegicus
native enzyme from pituitary to homogeneity from pituitary gland by 4-aminobenzoyl-Arg affinity chromatography with elution at pH 8.0 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal medulla
-
 Bos taurus
-
brain
-
 Rattus norvegicus
-
neuroendocrine cell
-
 Mus musculus
-
neuroendocrine cell
-
 Homo sapiens
-
neuroendocrine cell
-
 Rattus norvegicus
-
neuroendocrine cell
-
 Bos taurus
-
pancreas
-
 Mus musculus
-
pancreatic beta cell
-
 Mus musculus
-
pituitary gland
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Tyr-Ala-Arg + H2O
-
 Mus musculus acetyl-Tyr-Ala + Arg
-
 ?
acetyl-Tyr-Ala-Arg + H2O
-
 Homo sapiens acetyl-Tyr-Ala + Arg
-
 ?
acetyl-Tyr-Ala-Arg + H2O
-
 Rattus norvegicus acetyl-Tyr-Ala + Arg
-
 ?
acetyl-Tyr-Ala-Arg + H2O
-
 Bos taurus acetyl-Tyr-Ala + Arg
-
 ?
acetyl-Tyr-Ala-Arg + H2O
-
 Aplysia sp. acetyl-Tyr-Ala + Arg
-
 ?
benzoyl-Phe-Ala-Arg + H2O
-
 Mus musculus benzoyl-Phe-Ala + Arg
-
 ?
benzoyl-Phe-Ala-Arg + H2O
-
 Homo sapiens benzoyl-Phe-Ala + Arg
-
 ?
benzoyl-Phe-Ala-Arg + H2O
-
 Rattus norvegicus benzoyl-Phe-Ala + Arg
-
 ?
benzoyl-Phe-Ala-Arg + H2O
-
 Bos taurus benzoyl-Phe-Ala + Arg
-
 ?
benzoyl-Phe-Ala-Arg + H2O
-
 Aplysia sp. benzoyl-Phe-Ala + Arg
-
 ?
Dansyl-Phe-Ala-Arg + H2O
-
 Mus musculus Dansyl-Phe-Ala + Arg
-
 ?
Dansyl-Phe-Ala-Arg + H2O
-
 Homo sapiens Dansyl-Phe-Ala + Arg
-
 ?
Dansyl-Phe-Ala-Arg + H2O
-
 Rattus norvegicus Dansyl-Phe-Ala + Arg
-
 ?
Dansyl-Phe-Ala-Arg + H2O
-
 Bos taurus Dansyl-Phe-Ala + Arg
-
 ?
Dansyl-Phe-Ala-Arg + H2O
-
 Aplysia sp. Dansyl-Phe-Ala + Arg
-
 ?
additional information the enzyme contributes to the sorting of proteins into the secretory pathway Aplysia sp. ?
-
 ?
additional information the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters Mus musculus ?
-
 ?
additional information the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters Homo sapiens ?
-
 ?
additional information the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters Rattus norvegicus ?
-
 ?
additional information the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters Bos taurus ?
-
 ?
additional information the enzyme removes C-terminal basic residues, Arg, Lys, and to a minor extent also His, from a variety of substrates, but shows no activity against nonbasic residues, the enzyme prefers Met at the P1 position but tolerates all amino acids, showing low activity in cleaving Pro-Arg bonds, it has a preference for tri- and tetrapeptides compared to dipeptides Mus musculus ?
-
 ?
additional information the enzyme removes C-terminal basic residues, Arg, Lys, and to a minor extent also His, from a variety of substrates, but shows no activity against nonbasic residues, the enzyme prefers Met at the P1 position but tolerates all amino acids, showing low activity in cleaving Pro-Arg bonds, it has a preference for tri- and tetrapeptides compared to dipeptides Homo sapiens ?
-
 ?
additional information the enzyme removes C-terminal basic residues, Arg, Lys, and to a minor extent also His, from a variety of substrates, but shows no activity against nonbasic residues, the enzyme prefers Met at the P1 position but tolerates all amino acids, showing low activity in cleaving Pro-Arg bonds, it has a preference for tri- and tetrapeptides compared to dipeptides Rattus norvegicus ?
-
 ?
additional information the enzyme removes C-terminal basic residues, Arg, Lys, and to a minor extent also His, from a variety of substrates, but shows no activity against nonbasic residues, the enzyme prefers Met at the P1 position but tolerates all amino acids, showing low activity in cleaving Pro-Arg bonds, it has a preference for tri- and tetrapeptides compared to dipeptides Bos taurus ?
-
 ?
additional information the enzyme removes C-terminal basic residues, Arg, Lys, and to a minor extent also His, from a variety of substrates, but shows no activity against nonbasic residues, the enzyme prefers Met at the P1 position but tolerates all amino acids, showing low activity in cleaving Pro-Arg bonds, it has a preference for tri- and tetrapeptides compared to dipeptides Aplysia sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52000-56000 Rattus norvegicus
? x * 52000-56000 Bos taurus
More structure overview Mus musculus
More structure overview Homo sapiens
More structure overview Rattus norvegicus
More structure overview Bos taurus
More structure overview Aplysia sp.

Synonyms

Synonyms Comment Organism
carboxypeptidase H
-
 Bos taurus
CPE
-
 Mus musculus
CPE
-
 Homo sapiens
CPE
-
 Rattus norvegicus
CPE
-
 Bos taurus
CPE
-
 Aplysia sp.
enkephalin convertase formerly Bos taurus
More the enzyme belongs to the family of metallocarboxypeptidases Mus musculus
More the enzyme belongs to the family of metallocarboxypeptidases Homo sapiens
More the enzyme belongs to the family of metallocarboxypeptidases Rattus norvegicus
More the enzyme belongs to the family of metallocarboxypeptidases Bos taurus
More the enzyme belongs to the family of metallocarboxypeptidases Aplysia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
 Mus musculus
5 5.5
-
 Homo sapiens
5 5.5
-
 Rattus norvegicus
5 5.5
-
 Bos taurus
5 5.5
-
 Aplysia sp.

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 rapid decline in activity at pH values above pH 5.5 Mus musculus
additional information
-
 rapid decline in activity at pH values above pH 5.5 Homo sapiens
additional information
-
 rapid decline in activity at pH values above pH 5.5 Rattus norvegicus
additional information
-
 rapid decline in activity at pH values above pH 5.5 Bos taurus
additional information
-
 rapid decline in activity at pH values above pH 5.5 Aplysia sp.

pI Value

Organism Comment pI Value Maximum pI Value
Mus musculus about
-
 5
Homo sapiens about
-
 5
Rattus norvegicus about
-
 5
Bos taurus about
-
 5
Aplysia sp. about
-
 5