Application | Comment | Organism |
---|---|---|
biotechnology | Kex-1-C611 is proposed as a convenient biotechnological reagent for the cleavage of fusion proteins | Kluyveromyces lactis |
Cloned (Comment) | Organism |
---|---|
a truncated form of Kex-1, Kex-1-C611, is constructed comprising the amino acid residues from 104-611 and lacks the transmembrane domain | Kluyveromyces lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Kluyveromyces lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
calculated, truncated Kex-1-C611 | Kluyveromyces lactis |
67000 | - |
determined by SDS-PAGE, truncated Kex-1-C611 | Kluyveromyces lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Kluyveromyces lactis | preferential release of a C-terminal arginine or lysine residue | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces lactis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
archieved by a two-step chromatographic process using cation-exchange, SP Sepharose, followed by anion-exchange, DEAE Sepharose FF resin | Kluyveromyces lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
60 U/ml, purified truncated Kex-1-C611 | Kluyveromyces lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | preferential release of a C-terminal arginine or lysine residue | Kluyveromyces lactis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Kex-1 endopeptidase | - |
Kluyveromyces lactis |
Kex-1 protease | - |
Kluyveromyces lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
cleavage assay | Kluyveromyces lactis |