Cloned (Comment) | Organism |
---|---|
individual domains of the enzyme are expressed in insect Sf9 cells using the baculovirus expression system. Medium from domain 1B-expressing cells and domain 2-expressing cells shows substantial enzymatic activity, whereas medium from domain 1A-expressing cells is not different from cells infected with wild-type virus. The individual domains 1A, 1B, and 2 are expressed in baculovirus under the polyhedrin promoter and with the signal peptide of the rat enzyme | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
E353Q | cells infected with the mutant enzyme show considerable carboxypeptidase activity, mutation eliminates the activity of domain 1 | Drosophila melanogaster |
E771Q | cells infected with the mutant enzyme show considerable carboxypeptidase activity, mutation eliminates the activity of domain 2 | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-[[(3,4-dinitrophenyl)carbonyl]amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid | - |
Drosophila melanogaster | |
EDTA | - |
Drosophila melanogaster | |
Guanidinoethylmercaptosuccinic acid | domain 1B and domain | Drosophila melanogaster | |
PCMB | domain 1B and domain | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.208 | - |
dansyl-Phe-Ala-Arg | pH 7.4, 37°C, domain 1B | Drosophila melanogaster | |
0.246 | - |
dansyl-Phe-Ala-Arg | pH 5.7, 37°C, domain 2 | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Drosophila melanogaster | the enzyme functions in processing of proteins that transit the secretory pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
domain 1B and 2 expressed in Sf9 cells using baculovirus expression system | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Dansyl-Phe-Ala-Arg + H2O | - |
Drosophila melanogaster | Dansyl-Phe-Ala + Arg | - |
? | |
additional information | activity of individual domains of the enzyme. Domain 1B is more active at neutral pH and greatly prefers C-terminal Arg over Lys, whereas domain 2 is more active at pH 5-6 and slightly prefers C-terminal Lys over Arg | Drosophila melanogaster | ? | - |
? | |
additional information | the enzyme functions in processing of proteins that transit the secretory pathway | Drosophila melanogaster | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.86 | - |
dansyl-Phe-Ala-Arg | pH 5.7, 37°C, domain 2 | Drosophila melanogaster | |
32.6 | - |
dansyl-Phe-Ala-Arg | pH 7.4, 37°C, domain 1B | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | wild-type enzyme | Drosophila melanogaster |
5.6 | - |
domain 1B | Drosophila melanogaster |
7.4 | - |
domain 2 | Drosophila melanogaster |