Literature summary for 3.4.15.6 extracted from

Ponndorf, D.; Ehmke, S.; Walliser, B.; Thoss, K.; Unger, C.; Goers, S.; Das, G.; Metges, C.C.; Broer, I.; Nausch, H.
Stable production of cyanophycinase in Nicotiana benthamiana and its functionality to hydrolyse cyanophycin in the murine intestine (2017), Plant Biotechnol. J., 15, 605-613 .

Search for more literature for 3.4.15.6

Application

Application	Comment	Organism
nutrition	Nicotiana benthamiana-expressed cyanophycin fed in combination with Nicotiana benthamiana-expressed CGPase is hydrolysed in the intestine of mice, and high levels of beta-Asp-Arg dipeptides are found in plasma. However, there is no increase in Asp and Arg or its metabolite ornithine in plasma. Intestinal degradation of cyanophycin by CGPase leads to low intestinal cyanophycin content 4 h after consumption, but after ingestion of cyanophycin alone, high cyanophycin concentrations remain in the large intestine	Thermosynechococcus vestitus

Protein Variants

Protein Variants	Comment	Organism
additional information	optimized transient expression in Nicotiana benthamiana plants to produce high amounts of active CGPase. Protein stability is increased by the translational fusion of CGPase to the green fluorescent protein or to the transit peptide of the small subunit of RuBisCO for peptide production in the chloroplasts	Thermosynechococcus vestitus

Organism

Organism	UniProt	Comment	Textmining
Thermosynechococcus vestitus	P0C8P3	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Synonyms

Synonyms	Comment	Organism
CphB	-	Thermosynechococcus vestitus

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Release 2023.1 (January 2023)