Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli XL1-Blue | Pseudomonas anguilliseptica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 49% inhibition at 60 nM, 46% inhibition at 30 nM | Pseudomonas anguilliseptica | |
leupeptin | 13% inhibition within the range 0.001 nM to 0.01 nM | Pseudomonas anguilliseptica | |
N-bromo-succinimide | 100% inhibition at 5 nM, 99% inhibition at 1 nM | Pseudomonas anguilliseptica | |
pefabloc | i.e. 4-[2-aminoethyl]benzenesulfonyl fluoride, 92% inhibition at 80 nM, 13.6% inhibition at 8 nM | Pseudomonas anguilliseptica | |
phenylmethylsulfonyl fluoride | 63% inhibition at 10 nM, 28% inhibition at 1 nM | Pseudomonas anguilliseptica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas anguilliseptica | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
monomer, SDS-PAGE | Pseudomonas anguilliseptica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[L-Asp(4-Arg)]n + H2O | Pseudomonas anguilliseptica | - |
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)] | - |
? | |
[L-Asp(4-Arg)]n + H2O | Pseudomonas anguilliseptica BI | - |
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas anguilliseptica | Q8KQN8 | - |
- |
Pseudomonas anguilliseptica BI | Q8KQN8 | - |
- |
Purification (Comment) | Organism |
---|---|
from culture supernatant of Pseudomonas anguilliseptica cells grown in medium containing cyanophycin granules, anion-exchange chromatography and L-arginine-agarose affinity chromatography. In case of heterologous expression in Escherichia coli the enzyme was not secreted and thus, isolation was done from the cell lysate | Pseudomonas anguilliseptica |
Renatured (Comment) | Organism |
---|---|
in-gel renaturation method | Pseudomonas anguilliseptica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | cells grown in medium containing cyanophycin granules | Pseudomonas anguilliseptica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[L-Asp(4-Arg)]n + H2O | - |
Pseudomonas anguilliseptica | [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)] | - |
? | |
[L-Asp(4-Arg)]n + H2O | - |
Pseudomonas anguilliseptica BI | [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CGPase | - |
Pseudomonas anguilliseptica |
CphE | - |
Pseudomonas anguilliseptica |
cyanophycin granule polypeptidase | - |
Pseudomonas anguilliseptica |
cyanophycinase | - |
Pseudomonas anguilliseptica |
extracellular CGPase | - |
Pseudomonas anguilliseptica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas anguilliseptica | calculated from the sequence | - |
5.92 |