Crystallization (Comment) | Organism |
---|---|
purified enzyme, hanging drop method, mixing of 0.002 ml of protein solution containing 10 mg/ml protein in 1 mM 2-mercaptoethanol, 0.1 mM MnCl2, and 10 mM Tris-HCl, pH 7.2, with 0.002 ml of reservoir solution containing 16% PEG 4000, 20 mM MnCl2, 1 mM 2-mercaptoethanol, 270 mM ammonium acetate, and 60 mM sodium acetate, pH 4.6, at 20°C, 2-4 days, X-ray diffraction structure determination and analysis | Alteromonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N,N'-diisopropyldiamidofluorophosphate | i.e. DDFP or mipafox | Alteromonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the enzyme harbors binuclear Mn2+ ions within the active site | Alteromonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alteromonas sp. | - |
- |
- |
Alteromonas sp. JD6.5 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the bifunctional enzyme is active on the nerve agent organophosphate substrate diisopropyl fluorophosphate, DFP, producing N,N'-diisopropyldiamidophosphate, binding structure modelling, overview | Alteromonas sp. | ? | - |
? | |
additional information | the bifunctional enzyme is active on the nerve agent organophosphate substrate diisopropyl fluorophosphate, DFP, producing N,N'-diisopropyldiamidophosphate, binding structure modelling, overview | Alteromonas sp. JD6.5 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | The OPAA structure is composed of two domains, amino and carboxy domains, with the latter exhibiting a pita bread architecture and harboring the active site with the binuclear Mn2+ ions | Alteromonas sp. |
Synonyms | Comment | Organism |
---|---|---|
OPAA | - |
Alteromonas sp. |
organophosphate acid anhydrolase | - |
Alteromonas sp. |
organophosphate anhydrolase/prolidase | - |
Alteromonas sp. |
prolidase | - |
Alteromonas sp. |