Literature summary for 3.4.13.22 extracted from

Lv, M.; Zhang, Y.J.; Zhou, F.; Ge, Y.; Zhao, M.H.; Liu, Y.; Yang, K.W.
Real-time monitoring of D-Ala-D-Ala dipeptidase activity of VanX in living bacteria by isothermal titration calorimetry (2019), Anal. Biochem., 578, 29-35 .

Search for more literature for 3.4.13.22

Application

Application	Comment	Organism
analysis	continuous assay of VanX in vitro and in vivo from hydrolysis of D-Ala-D-Ala, based on the heat-rate changes measured with isothermal titration calorimetry	Enterococcus faecalis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and clinical strain VRE	Enterococcus faecalis

Inhibitors

Inhibitors	Comment	Organism	Structure
D-cysteine	-	Enterococcus faecalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	D-Ala-D-Ala	pH 8.0, 37°C	Enterococcus faecalis

Organism

Organism	UniProt	Comment	Textmining
Enterococcus faecalis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Ala-D-Ala + H2O	-	Enterococcus faecalis	2 D-Ala	-	?

Synonyms

Synonyms	Comment	Organism
VanX	-	Enterococcus faecalis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
11.5	-	D-Ala-D-Ala	pH 8.0, 37°C	Enterococcus faecalis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0188	-	D-cysteine	pH 8.0, 37°C	Enterococcus faecalis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0298	-	pH 8.0, 37°C	Enterococcus faecalis	D-cysteine

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Release 2023.1 (January 2023)