Literature summary for 3.4.11.5 extracted from
Wang, Y.; Liu, H.; Wang, S.; Li, H.; Xin, Q.
Overexpressing of a novel wheat prolyl aminopeptidase gene enhances zinc stress tolerance in transgenic Arabidopsis thaliana (2015), Plant Cell, 121, 489-499 .
No PubMed abstract available
Application
Application |
Comment |
Organism |
agriculture |
potential use of the enzyme in the transgenic breeding to improve heavy metal resistance in crop species |
Triticum aestivum |
biotechnology |
potential use of the enzyme in the transgenic breeding to improve heavy metal resistance in crop species |
Triticum aestivum |
Cloned(Commentary)
Cloned (Comment) |
Organism |
PAP1, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression of the enzyme in transgenic Arabidopsis thaliana plants under control of CaMV 35S promoter via the Agrobacterium tumefaciens strain GV3101 transfection system, real-time PCR enzyme expression analysis, recombinant expression as GFP-tagged enzyme in the cytoplasm |
Triticum aestivum |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
overexpression of enzyme TaPAP1 from Triticum aestivum in transgenic Arabidopsis thaliana plants, zinc-stressed TaPAP1 transgenic Arabidopsis displays higher survival rate, fresh weight, photosynthetic efficiency, proline levels, and PAP activity compared to wild-type Arabidopsis thaliana plants |
Triticum aestivum |
Localization
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
cytoplasm |
protein TaPAP1 is localized mainly in the cytoplasm |
Triticum aestivum |
5737 |
- |
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Cu2+ |
induces the enzyme expression, overview |
Triticum aestivum |
|
Zn2+ |
induces the enzyme expression, overview |
Triticum aestivum |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Triticum aestivum |
A0A3B5Y450 |
cultivar Jinan 18 |
- |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
root |
TaPAP1 is predominantly expressed in seedling roots |
Triticum aestivum |
- |
seedling |
TaPAP1 is predominantly expressed in seedling roots |
Triticum aestivum |
- |
Subunits
Subunits |
Comment |
Organism |
? |
x * 43900, about, sequence calculation |
Triticum aestivum |
Synonyms
Synonyms |
Comment |
Organism |
Prolyl aminopeptidase |
- |
Triticum aestivum |
TaPAP1 |
- |
Triticum aestivum |
pI Value
Organism |
Comment |
pI Value Maximum |
pI Value |
Triticum aestivum |
sequence calculation |
- |
7.94 |
Expression
Organism |
Comment |
Expression |
Triticum aestivum |
the enzyme expression is upregulated by various abiotic stresses, such as salt, drought, cold, copper and zinc, overview |
up |
General Information
General Information |
Comment |
Organism |
evolution |
enzyme TsPAP1 belongs to the S33.001 subfamily of aminopeptidases |
Triticum aestivum |
physiological function |
TaPAP1 is involved in the plant response to zinc stress. Overexpressing of the Triticum aestivum prolyl aminopeptidase gene enhances zinc stress tolerance in transgenic Arabidopsis thaliana |
Triticum aestivum |