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Literature summary for 3.4.11.20 extracted from

  • Dalal, S.; Ragheb, D.R.; Klemba, M.
    Engagement of the S1, S1' and S2' subsites drives efficient catalysis of peptide bond hydrolysis by the M1-family aminopeptidase from Plasmodium falciparum (2012), Mol. Biochem. Parasitol., 183, 70-77.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 Leu-Gly-Leu pH 7.5, 30°C Plasmodium falciparum
0.2
-
 Leu-Gly-Pro pH 7.5, 30°C Plasmodium falciparum
0.4
-
 Arg-Ala pH 7.5, 30°C Plasmodium falciparum
0.42
-
 Leu-Gly-NH2 pH 7.5, 30°C Plasmodium falciparum
0.43
-
 Lys-Ala pH 7.5, 30°C Plasmodium falciparum
0.64
-
 Leu-Ala pH 7.5, 30°C Plasmodium falciparum
0.7
-
 Leu-Gly-Gly pH 7.5, 30°C Plasmodium falciparum
0.95
-
 Leu-Gly pH 7.5, 30°C Plasmodium falciparum
4.2
-
 His-Ala pH 7.5, 30°C Plasmodium falciparum
4.9
-
 Leu-NH2 pH 7.5, 30°C Plasmodium falciparum
8.9
-
 Lys-Ala pH 5.5, 30°C Plasmodium falciparum
9
-
 Arg-Ala pH 5.5, 30°C Plasmodium falciparum
10
-
 Leu-Ala pH 5.5, 30°C Plasmodium falciparum
35
-
 His-Ala pH 5.5, 30°C Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Ala + H2O
-
 Plasmodium falciparum L-Arg + L-Ala
-
 ?
His-Ala + H2O
-
 Plasmodium falciparum L-His + L-Ala
-
 ?
Leu-Ala + H2O
-
 Plasmodium falciparum L-Leu + L-Ala
-
 ?
Leu-Gly + H2O
-
 Plasmodium falciparum L-Leu + Gly
-
 ?
Leu-Gly-Gly + H2O
-
 Plasmodium falciparum ?
-
 ?
Leu-Gly-Leu + H2O
-
 Plasmodium falciparum ?
-
 ?
Leu-Gly-NH2 + H2O
-
 Plasmodium falciparum ?
-
 ?
Leu-Gly-Pro + H2O
-
 Plasmodium falciparum ?
-
 ?
Leu-NH2 + H2O
-
 Plasmodium falciparum L-Leu + NH3
-
 ?
Lys-Ala + H2O
-
 Plasmodium falciparum L-Lys + L-Ala
-
 ?
additional information both S1 and S1' subsite exhibit a preference for basic and hydrophobic side chains over polar and acidic side chains. The relative specificity of the S1 subsite is similar over the pH range 5.5-7.5. Substrate P1 and P1' residues affect both Km and kcat, revealing that side chain-subsite interactions not only drive the formation of the Michaelis complex but also influence the rates of ensuing chemical steps. There is no correlation between S1 and S1' specificities and amino acid abundance in hemoglobin. Interactions between PfA-M1 and the backbone atoms of the P1' and P2' residues as well as the P2' side chain further contribute to the catalytic efficiency of substrate hydrolysis Plasmodium falciparum ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 His-Ala pH 5.5, 30°C Plasmodium falciparum
2.4
-
 His-Ala pH 7.5, 30°C Plasmodium falciparum
4.8
-
 Leu-Gly-Leu pH 7.5, 30°C Plasmodium falciparum
6.5
-
 Leu-Gly pH 7.5, 30°C Plasmodium falciparum
9.3
-
 Leu-Ala pH 7.5, 30°C Plasmodium falciparum
9.5
-
 Arg-Ala pH 7.5, 30°C Plasmodium falciparum
12
-
 Leu-NH2 pH 7.5, 30°C Plasmodium falciparum
12
-
 Leu-Gly-Pro pH 7.5, 30°C Plasmodium falciparum
12
-
 Leu-Ala pH 5.5, 30°C Plasmodium falciparum
15
-
 Arg-Ala pH 5.5, 30°C Plasmodium falciparum
21
-
 Leu-Gly-Gly pH 7.5, 30°C Plasmodium falciparum
21
-
 Leu-Gly-NH2 pH 7.5, 30°C Plasmodium falciparum
27
-
 Lys-Ala pH 7.5, 30°C Plasmodium falciparum
27
-
 Lys-Ala pH 5.5, 30°C Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.027
-
 His-Ala pH 5.5, 30°C Plasmodium falciparum
0.56
-
 His-Ala pH 7.5, 30°C Plasmodium falciparum
1.2
-
 Leu-Ala pH 5.5, 30°C Plasmodium falciparum
1.7
-
 Arg-Ala pH 5.5, 30°C Plasmodium falciparum
2.4
-
 Leu-NH2 pH 7.5, 30°C Plasmodium falciparum
3
-
 Lys-Ala pH 5.5, 30°C Plasmodium falciparum
6.8
-
 Leu-Gly pH 7.5, 30°C Plasmodium falciparum
14
-
 Leu-Ala pH 7.5, 30°C Plasmodium falciparum
23
-
 Arg-Ala pH 7.5, 30°C Plasmodium falciparum
30
-
 Leu-Gly-Gly pH 7.5, 30°C Plasmodium falciparum
49
-
 Leu-Gly-NH2 pH 7.5, 30°C Plasmodium falciparum
60
-
 Leu-Gly-Pro pH 7.5, 30°C Plasmodium falciparum
65
-
 Lys-Ala pH 7.5, 30°C Plasmodium falciparum
330
-
 Leu-Gly-Leu pH 7.5, 30°C Plasmodium falciparum