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Literature summary for 3.4.11.20 extracted from

  • Gras, S.; Byzia, A.; Gilbert, F.B.; McGowan, S.; Drag, M.; Silvestre, A.; Niepceron, A.; Lecaille, F.; Lalmanach, G.; Brossier, F.
    Aminopeptidase N1 (EtAPN1), an M1 metalloprotease of the apicomplexan parasite Eimeria tenella, participates in parasite development (2014), Eukaryot. Cell, 13, 884-895.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline treatment with 1,10-ortho-phenanthroline in order to deplete metal ions, results in a residual activity of about 8% compared to the initial activity. Activity is partially restored by the addition of divalent metal cations with Zn2+ being the most potent Eimeria tenella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0165
-
norleucyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.0236
-
(S)-2-amino-4-cyclohexylbutanoyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.0262
-
homophenylalanyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.0277
-
homoargininyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.0302
-
styryl-Ala-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.0314
-
L-methionyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.0354
-
norvalinyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.0598
-
Ala-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ treatment with 1,10-phenanthroline in order to deplete metal ions, results in a residual activity of about 8% compared to the initial activity. Activity is partially restored by the addition of divalent metal cations with Zn2+ being the most potent Eimeria tenella

Organism

Organism UniProt Comment Textmining
Eimeria tenella V5T9T8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification isoform APN1 is synthesized as a 120-kDa precursor and cleaved into 96-, 68-, and 38-kDa forms during sporulation Eimeria tenella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-amino-4-cyclohexylbutanoyl-7-amido-4-methylcoumarin + H2O
-
Eimeria tenella (S)-2-amino-4-cyclohexylbutanoic acid + 7-amino-4-methylcoumarin
-
?
Ala-7-amido-4-methylcoumarin + H2O
-
Eimeria tenella alanine + 7-amino-4-methylcoumarin
-
?
homoargininyl-7-amido-4-methylcoumarin + H2O
-
Eimeria tenella homoarginine + 7-amino-4-methylcoumarin
-
?
homophenylalanyl-7-amido-4-methylcoumarin + H2O
-
Eimeria tenella homophenylalanine + 7-amino-4-methylcoumarin
-
?
L-methionyl-7-amido-4-methylcoumarin + H2O
-
Eimeria tenella L-methionine + 7-amino-4-methylcoumarin
-
?
norleucyl-7-amido-4-methylcoumarin + H2O
-
Eimeria tenella norleucine + 7-amino-4-methylcoumarin
-
?
norvalinyl-7-amido-4-methylcoumarin + H2O
-
Eimeria tenella norvaline + 7-amino-4-methylcoumarin
-
?
styryl-Ala-7-amido-4-methylcoumarin + H2O
-
Eimeria tenella styryl-alanine + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
APN1
-
Eimeria tenella

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.456
-
Ala-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.575
-
homoargininyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.577
-
styryl-Ala-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.658
-
norvalinyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.704
-
L-methionyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.71
-
norleucyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.737
-
(S)-2-amino-4-cyclohexylbutanoyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
0.836
-
homophenylalanyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella

Expression

Organism Comment Expression
Eimeria tenella isoform APN1 is present during the intracellular life cycle stages in both the parasite cytoplasm and the parasite nucleus additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.6
-
Ala-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
18.6
-
norvalinyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
19.1
-
styryl-Ala-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
20.8
-
homoargininyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
22.4
-
L-methionyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
31.9
-
homophenylalanyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella
43
-
norleucyl-7-amido-4-methylcoumarin pH 7.4, 37┬░C Eimeria tenella