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Literature summary for 3.4.11.2 extracted from
- Leucine aminopeptidase may contribute to the intrinsic resistance of cancer cells toward cisplatin as revealed by an ultrasensitive fluorescent probe (2016), Chem. Sci., 7, 788-792 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | inhibition of expression of LAP with siRNA | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ubenimex | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0467 | - |
9-(L-leucylamino)-5H-benzo[a]phenoxazin-5-iminium | 37°C, pH 7.4 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P28838 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| A-549 cell | - |
Homo sapiens | - |
| carcinoma cell | - |
Homo sapiens | - |
| Hep-G2 cell | - |
Homo sapiens | - |
| liver | fluorometric detection of LAP in human liver microsomes of cancer cells | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9-(L-leucylamino)-5H-benzo[a]phenoxazin-5-iminium + H2O | the substrate displays high selectivity for LAP over various other substances (including biothiols and co-existing enzymes) at their physiological concentrations. It is an excellent specific substrate for enzyme LAP | Homo sapiens | 9-amino-5H-benzo[a]phenoxazin-5-iminium + L-leucine | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LAP | - |
Homo sapiens |
| lap3 | - |
Homo sapiens |
| leucine aminopeptidase | - |
Homo sapiens |
| microsomal leucine aminopeptidase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | inhibiting the expression of LAP with siRNA decreases cell viability | Homo sapiens |
| physiological function | intracellular LAP plays a role in the intrinsic drug resistance to cisplatin in cancer cells | Homo sapiens |
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Release 2023.1 (January 2023)
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