BRENDA - Enzyme Database
show all sequences of 3.4.11.13

A novel aminopeptidase from Clostridium histolyticum

Kessler, E.; Yaron, A.; Biochem. Biophys. Res. Commun. 50, 405-412 (1973) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Hathewaya histolytica
p-mercuribenzoate
-
Hathewaya histolytica
Zn2+
-
Hathewaya histolytica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Hathewaya histolytica
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Hathewaya histolytica
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cd2+
-
Hathewaya histolytica
Co2+
-
Hathewaya histolytica
Mn2+
-
Hathewaya histolytica
Ni2+
-
Hathewaya histolytica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
340000
-
gel filtration
Hathewaya histolytica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Peptides + H2O
Hathewaya histolytica
-
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Hathewaya histolytica
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Hathewaya histolytica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10.5
-
-
Hathewaya histolytica
Storage Stability
Storage Stability
Organism
-20°C, 0.05 M phosphate buffer or 0.05 M Na-acetate buffer, pH 5.6, 1 mM EDTA, several years, little loss of activity, can be restored by treatment with 2.5 mM dithiothreitol
Hathewaya histolytica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(Ala)3 + H2O
-
81197
Hathewaya histolytica
Ala + Ala-Ala
-
81197
Hathewaya histolytica
ir
(Ala)4 + H2O
-
81197
Hathewaya histolytica
Ala + (Ala)3
or Ala-Ala + Ala-Ala
81197
Hathewaya histolytica
ir
(Ala)4-NH2 + H2O
-
81197
Hathewaya histolytica
Ala + (Ala)3-NH2
or Ala-Ala + Ala-Ala-NH2 or (Ala)3 + Ala-NH2
81197
Hathewaya histolytica
ir
(Pro-Gly-Pro)4-6 + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-(Pro-Gly-Pro)3-5
-
81197
Hathewaya histolytica
ir
(Pro-Gly-Pro)4-6-OMe + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-(Pro-Gly-Pro)3-5-OMe
-
81197
Hathewaya histolytica
ir
Ala-Ala-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Ala + Ala-Phe-Ala
or Ala-Ala + Phe-Ala
81197
Hathewaya histolytica
ir
Ala-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Ala + Gly-Gly
-
81197
Hathewaya histolytica
ir
Ala-Lys + H2O
-
81197
Hathewaya histolytica
Ala + Lys
-
81197
Hathewaya histolytica
ir
Gly-Gly-Phe + H2O
-
81197
Hathewaya histolytica
Gly + Gly-Phe
-
81197
Hathewaya histolytica
ir
Gly-Gly-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Gly + Gly-Phe-Ala
or Gly-Gly + Phe-Ala
81197
Hathewaya histolytica
ir
Gly-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Gly + Phe-Ala
-
81197
Hathewaya histolytica
ir
Hyp-Ala-NH2 + H2O
-
81197
Hathewaya histolytica
Hyp-Ala + NH3
-
81197
Hathewaya histolytica
ir
Leu-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Leu + Gly-Gly
-
81197
Hathewaya histolytica
ir
Met-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Met + Gly-Gly
-
81197
Hathewaya histolytica
ir
Met-Met + H2O
-
81197
Hathewaya histolytica
Met + Met
-
81197
Hathewaya histolytica
ir
Peptides + H2O
-
81197
Hathewaya histolytica
?
-
-
-
?
Phe-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Phe + Gly-Gly
-
81197
Hathewaya histolytica
ir
Pro-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Gly
-
81197
Hathewaya histolytica
ir
Pro-Gly-Pro + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro
-
-
-
?
Pro-Gly-Pro-Pro + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-Pro
-
81197
Hathewaya histolytica
ir
Pro-Leu + H2O
-
81197
Hathewaya histolytica
Pro + Leu
-
81197
Hathewaya histolytica
ir
Pro-Phe + H2O
-
81197
Hathewaya histolytica
Pro + Phe
-
81197
Hathewaya histolytica
ir
Ser-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Ser + Gly-Gly
-
81197
Hathewaya histolytica
ir
Val-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Val + Gly-Gly
-
81197
Hathewaya histolytica
ir
Subunits
Subunits
Commentary
Organism
hexamer
6 * 51000-59000, SDS-PAGE
Hathewaya histolytica
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Hathewaya histolytica
276
-
Val-Gly-Gly
-
Hathewaya histolytica
337
-
Pro-Gly-Pro-Pro
-
Hathewaya histolytica
561
-
Pro-Gly-Pro
-
Hathewaya histolytica
667
-
Phe-Gly-Gly
-
Hathewaya histolytica
917
-
Leu-Gly-Gly
-
Hathewaya histolytica
1360
-
Pro-Gly-Gly
-
Hathewaya histolytica
1830
-
Ala-Gly-Gly
-
Hathewaya histolytica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
assay at
Hathewaya histolytica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Hathewaya histolytica
p-mercuribenzoate
-
Hathewaya histolytica
Zn2+
-
Hathewaya histolytica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Hathewaya histolytica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Hathewaya histolytica
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cd2+
-
Hathewaya histolytica
Co2+
-
Hathewaya histolytica
Mn2+
-
Hathewaya histolytica
Ni2+
-
Hathewaya histolytica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
340000
-
gel filtration
Hathewaya histolytica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Peptides + H2O
Hathewaya histolytica
-
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Hathewaya histolytica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10.5
-
-
Hathewaya histolytica
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 0.05 M phosphate buffer or 0.05 M Na-acetate buffer, pH 5.6, 1 mM EDTA, several years, little loss of activity, can be restored by treatment with 2.5 mM dithiothreitol
Hathewaya histolytica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(Ala)3 + H2O
-
81197
Hathewaya histolytica
Ala + Ala-Ala
-
81197
Hathewaya histolytica
ir
(Ala)4 + H2O
-
81197
Hathewaya histolytica
Ala + (Ala)3
or Ala-Ala + Ala-Ala
81197
Hathewaya histolytica
ir
(Ala)4-NH2 + H2O
-
81197
Hathewaya histolytica
Ala + (Ala)3-NH2
or Ala-Ala + Ala-Ala-NH2 or (Ala)3 + Ala-NH2
81197
Hathewaya histolytica
ir
(Pro-Gly-Pro)4-6 + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-(Pro-Gly-Pro)3-5
-
81197
Hathewaya histolytica
ir
(Pro-Gly-Pro)4-6-OMe + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-(Pro-Gly-Pro)3-5-OMe
-
81197
Hathewaya histolytica
ir
Ala-Ala-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Ala + Ala-Phe-Ala
or Ala-Ala + Phe-Ala
81197
Hathewaya histolytica
ir
Ala-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Ala + Gly-Gly
-
81197
Hathewaya histolytica
ir
Ala-Lys + H2O
-
81197
Hathewaya histolytica
Ala + Lys
-
81197
Hathewaya histolytica
ir
Gly-Gly-Phe + H2O
-
81197
Hathewaya histolytica
Gly + Gly-Phe
-
81197
Hathewaya histolytica
ir
Gly-Gly-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Gly + Gly-Phe-Ala
or Gly-Gly + Phe-Ala
81197
Hathewaya histolytica
ir
Gly-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Gly + Phe-Ala
-
81197
Hathewaya histolytica
ir
Hyp-Ala-NH2 + H2O
-
81197
Hathewaya histolytica
Hyp-Ala + NH3
-
81197
Hathewaya histolytica
ir
Leu-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Leu + Gly-Gly
-
81197
Hathewaya histolytica
ir
Met-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Met + Gly-Gly
-
81197
Hathewaya histolytica
ir
Met-Met + H2O
-
81197
Hathewaya histolytica
Met + Met
-
81197
Hathewaya histolytica
ir
Peptides + H2O
-
81197
Hathewaya histolytica
?
-
-
-
?
Phe-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Phe + Gly-Gly
-
81197
Hathewaya histolytica
ir
Pro-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Gly
-
81197
Hathewaya histolytica
ir
Pro-Gly-Pro + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro
-
-
-
?
Pro-Gly-Pro-Pro + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-Pro
-
81197
Hathewaya histolytica
ir
Pro-Leu + H2O
-
81197
Hathewaya histolytica
Pro + Leu
-
81197
Hathewaya histolytica
ir
Pro-Phe + H2O
-
81197
Hathewaya histolytica
Pro + Phe
-
81197
Hathewaya histolytica
ir
Ser-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Ser + Gly-Gly
-
81197
Hathewaya histolytica
ir
Val-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Val + Gly-Gly
-
81197
Hathewaya histolytica
ir
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 * 51000-59000, SDS-PAGE
Hathewaya histolytica
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Hathewaya histolytica
276
-
Val-Gly-Gly
-
Hathewaya histolytica
337
-
Pro-Gly-Pro-Pro
-
Hathewaya histolytica
561
-
Pro-Gly-Pro
-
Hathewaya histolytica
667
-
Phe-Gly-Gly
-
Hathewaya histolytica
917
-
Leu-Gly-Gly
-
Hathewaya histolytica
1360
-
Pro-Gly-Gly
-
Hathewaya histolytica
1830
-
Ala-Gly-Gly
-
Hathewaya histolytica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
assay at
Hathewaya histolytica
Other publictions for EC 3.4.11.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665095
Woessner
-
Clostridial aminopeptidase ...
Hathewaya histolytica
Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds)
2nd. Ed.
1011-1012
2004
-
-
-
-
-
1
4
-
-
2
2
-
-
1
-
-
1
-
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
4
1
-
-
2
2
-
-
-
-
1
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
81193
Fleminger
Soluble and immobilized clostr ...
Hathewaya histolytica
Biochim. Biophys. Acta
789
245-256
1984
-
-
-
-
-
-
2
1
-
2
-
1
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
2
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
81194
Fleminger
Sequential hydrolysis of proli ...
Hathewaya histolytica
Biochim. Biophys. Acta
743
437-446
1983
-
1
-
-
-
2
-
1
-
2
-
1
-
1
-
-
-
-
-
-
1
1
4
-
-
1
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
1
-
2
-
1
-
-
-
-
-
-
1
1
4
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
81195
Kessler
An extracellular aminopeptidas ...
Hathewaya histolytica
Eur. J. Biochem.
63
271-287
1976
-
-
-
-
-
-
3
8
1
4
1
1
-
1
-
-
1
-
-
-
1
1
25
1
-
1
-
-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
8
1
4
1
1
-
-
-
1
-
-
1
1
25
1
1
-
-
7
2
-
-
-
-
-
-
-
-
-
81196
Kessler
Extracellular aminopeptidase f ...
Hathewaya histolytica
Methods Enzymol.
45
544-552
1976
-
-
-
-
-
1
3
7
1
4
1
1
-
1
-
-
1
-
-
-
1
1
24
1
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
7
1
4
1
1
-
-
-
1
-
-
1
1
24
1
1
-
-
7
-
-
-
-
-
-
-
-
-
-
81197
Kessler
A novel aminopeptidase from Cl ...
Hathewaya histolytica
Biochem. Biophys. Res. Commun.
50
405-412
1973
-
-
-
-
-
-
3
1
1
4
1
1
-
1
-
-
1
-
-
-
1
1
24
1
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
1
4
1
1
-
-
-
1
-
-
1
1
24
1
-
-
-
8
1
-
-
-
-
-
-
-
-
-