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Literature summary for 3.3.2.6 extracted from

  • Rybina, I.V.; Feinmark, S.J.
    Alteration of human leukotriene A4 hydrolase activity after site-directed mutagenesis: serine-415 is a regulatory residue (1999), Biochim. Biophys. Acta, 1438, 199-203.
    View publication on PubMed
Search for more literature for 3.3.2.6

Application

Application Comment Organism
medicine the lipid metabolic activity of enzyme plays a central role in the control of polymorphonuclear leukocyte function and in the development of inflammation, the regulation of enzyme presents a novel potential target for anti-inflammatory therapy Homo sapiens
medicine the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
S415D significantly reduced epoxide hydrolase activity, little effect on aminopeptidase activity Homo sapiens
S415E significantly reduced epoxide hydrolase activity, little effect on aminopeptidase activity Homo sapiens
S415Q no effect on enzyme activity Homo sapiens
S415T little effect on enzyme activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 x * 69000, SDS-PAGE, mutant enzymes   Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
leukotriene A4 + H2O Homo sapiens biosynthesis of leukotriene B4 leukotriene B4
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
leukotriene A4 + H2O = leukotriene B4 enzyme contains two partly overlapping active sites which influence each other Homo sapiens
a
leukotriene A4 + H2O = leukotriene B4 Ser415 plays a key role in regulating epoxid hydrolase activity Homo sapiens
a
leukotriene A4 + H2O = leukotriene B4 bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
epithelium inactive form of enzyme Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
leukotriene A4 + H2O
-
 Homo sapiens leukotriene B4
-
 ?
leukotriene A4 + H2O biosynthesis of leukotriene B4 Homo sapiens leukotriene B4
-
 ?

Subunits

Subunits Comment Organism
? x * 69000, SDS-PAGE, mutant enzymes   Homo sapiens