Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces hygroscopicus subsp. ascomyceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
chorismate | in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20°C | Streptomyces hygroscopicus subsp. ascomyceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + H2O | Streptomyces hygroscopicus subsp. ascomyceticus | - |
(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus subsp. ascomyceticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Streptomyces hygroscopicus subsp. ascomyceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + H2O | - |
Streptomyces hygroscopicus subsp. ascomyceticus | (4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fkbO | - |
Streptomyces hygroscopicus subsp. ascomyceticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.6 | - |
chorismate | in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20°C | Streptomyces hygroscopicus subsp. ascomyceticus |