Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofuran-containing DNA dublex | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamic characterization of Fpg binding to lesion-containing 13mer DNA dublexes, binding affinity | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | maximal efficiency at 100 mM NaCl | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Escherichia coli | bifunctional base excision repair enzyme: DNA glycosylase/AP lyase | ? | - |
? | |
DNA + H2O | Escherichia coli B834 (DE3) | bifunctional base excision repair enzyme: DNA glycosylase/AP lyase | ? | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | Escherichia coli | natural substrate: 7,8-dihydro-8-oxo-dG, DNA base excision repair | 8-hydroxyguanine + DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
harboring PET13a-fpg plasmid | - |
Escherichia coli B834 (DE3) | - |
harboring PET13a-fpg plasmid | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine | mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions | Escherichia coli | ? | - |
? | |
DNA + H2O | FpG-DNA interactions establish contacts with DNA ligands, which span no more than 9 base-pairs, structural studies of Fpg-DNA complexes | Escherichia coli | ? | - |
? | |
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli | ? | - |
? | |
DNA + H2O | bifunctional base excision repair enzyme: DNA glycosylase/AP lyase | Escherichia coli | ? | - |
? | |
DNA + H2O | with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions | Escherichia coli B834 (DE3) | ? | - |
? | |
DNA + H2O | FpG-DNA interactions establish contacts with DNA ligands, which span no more than 9 base-pairs, structural studies of Fpg-DNA complexes | Escherichia coli B834 (DE3) | ? | - |
? | |
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli B834 (DE3) | ? | - |
? | |
DNA + H2O | bifunctional base excision repair enzyme: DNA glycosylase/AP lyase | Escherichia coli B834 (DE3) | ? | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | DNA glycosylase/AP lyase activity | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | 7,8-dihydro-8-oxo-2-deoxyguanosine, natural substrate, substrate recognition, mechanism | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | 7,8-dihydro-8-oxoguanine-DNA | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | natural substrate: 7,8-dihydro-8-oxo-dG, DNA base excision repair | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
additional information | not: carbocyclic substrate analog of 8-oxo-7,8-dihydro-2-deoxyguanosine | Escherichia coli | ? | - |
? | |
additional information | not: carbocyclic substrate analog of 8-oxo-7,8-dihydro-2-deoxyguanosine | Escherichia coli B834 (DE3) | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
free protein undergoes irreversible thermal unfolding with a Tm of about 65°C | Escherichia coli |