Protein Variants | Comment | Organism |
---|---|---|
C171A | does not infer with enzymatic depurination activity, leaving C259 as docking station for the fluorescence dye, which changes its emission properties upon change from aqueous to hydrophobic (membrane) environment, C259 is reduced in the lumen of the endoplasmic reticulum | Ricinus communis |
E177D/C259S/I249C | residues 249 and 259 show membrane- and temperature-induced structural transition, meaning that the residues are exposed to the bilayer interior, both labeled with N,N'-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine | Ricinus communis |
K4R/C171A/E177D/K239R/E135K | no temperature-induced change, meaning that residue 135 is bound within the membrane right away, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate | Ricinus communis |
K4R/C171A/E177D/K239R/E61K | no temperature-induced change, meaning that residue 61 is bound within the membrane right away, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate | Ricinus communis |
K4R/C171A/E177D/K239R/Q128K | no temperature-induced change, meaning that residue 128 is bound within the membrane right away, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate | Ricinus communis |
K4R/C171A/E177D/K239R/Q98K | no temperature-induced change, meaning that residue 98 is bound within the membrane right away, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate | Ricinus communis |
K4R/C171A/E177D/K239R/R114K | no temperature-induced change, meaning that residue 114 is bound within the membrane right away, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate | Ricinus communis |
K4R/C171A/E177D/K239R/R31K | residues 31 shows membrane- and temperature-induced structural transition, meaning that the residues are exposed to the bilayer interior, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate | Ricinus communis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | stably membrane-bound at 37°C, even under sodium carbonate treatment at pH 11.5, extractable at 20°C | Ricinus communis | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | temperature induced major structural rearrangements of endoplasmic reticulum membrane-bound toxin at 37°C: loss of helical content, increase of beta sheets, C-terminus moves closer to membrane surface, insertion into the bilayer | Ricinus communis |
Purification (Comment) | Organism |
---|---|
according to Deeks et al., Biochemistry 41, p. 3405-3413, 2002 | Ricinus communis |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rRNA + H2O | - |
Ricinus communis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ricin toxin A-chain | - |
Ricinus communis |
RTA | - |
Ricinus communis |