Literature summary for 3.2.1.B28 extracted from

Lin, X.A.; Zhang, W.; Chen, Y.; Yao, B.; Zhang, Z.F.
Overexpression of celB gene coding for beta-glucosidase from Pyrococcus furiosus using a baculovirus expression vector system in silkworm, Bombyx mori (2006), Z. Naturforsch. C, 61, 595-600.

Search for more literature for 3.2.1.B28

Application

Application	Comment	Organism
synthesis	expression of enzyme using a baculovirus expression vector system in silkworm, Bombyx mori and purification to about 81% homogeneity in a single heat-treatment step. The expressed beta-glucosidase accounts for more than 10% of silkworm total haemolymph proteins. The expression level reaches 10199.5 U per ml hemolymph and 19797.4 U per silkworm larva, and the specific activity of the one-step purified crude enzyme is 885 U per mg	Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment)	Organism
expression using a baculovirus expression vector system in silkworm, Bombyx mori	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	Q51723	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
885	-	recombinant protein, 90°C, pH 5.0	Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Pyrococcus furiosus	4-nitrophenol + beta-D-glucopyranose	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
105	-	recombinant protein	Pyrococcus furiosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	recombinant protein	Pyrococcus furiosus

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Release 2023.1 (January 2023)