KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46 | - |
pentyl beta-D-glucopyranoside | pH 5.0, 80°C, 6% water, 94% hexanol | Pyrococcus furiosus |
Organic Solvent | Comment | Organism |
---|---|---|
hexanol | enzyme catalyzes both hydrolysis and transglycosylation of glycosidc substrates. In hexanol/water two-phase systems, hydrolysis is by far the dominating reaction even though the total activity increases. In hexanol containing various amounts of water, the selectivity for the alcohol increases with increasing water activity. This counteracts the effect of higher water concentration and the transglycosylation/hydrolysis ratio increases with increasing water activity | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q51723 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
additional information | enzyme catalyzes both hydrolysis and transglycosylation of glycosidc substrates. In hexanol/water two-phase systems, hydrolysis is by far the dominating reaction even though the total activity increases. In hexanol containing various amounts of water, the selectivity for the alcohol increases with increasing water activity. This counteracts the effect of higher water concentration and the transglycosylation/hydrolysis ratio increases with increasing water activity | Pyrococcus furiosus | ? | - |
? | |
pentyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | pentanol + beta-D-glucopyranose | - |
? |