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Literature summary for 3.2.1.B28 extracted from

  • Yeom, S.J.; Kim, B.N.; Kim, Y.S.; Oh, D.K.
    Hydrolysis of isoflavone glycosides by a thermostable beta-glucosidase from Pyrococcus furiosus (2012), J. Agric. Food Chem., 60, 1535-1541.
    View publication on PubMed
Search for more literature for 3.2.1.B28

Activating Compound

Activating Compound Comment Organism Structure
additional information not activated by monovalent or divalent cations Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by EDTA Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 glycitin pH 6.0, 95°C Pyrococcus furiosus
0.42
-
 daidzin pH 6.0, 95°C Pyrococcus furiosus
0.5
-
 genistin pH 6.0, 95°C Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 4 * 56000, SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q51723
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
75
-
 substrate: glycitin, pH 6.0, 95°C Pyrococcus furiosus
180
-
 substrate: daidzin, pH 6.0, 95°C Pyrococcus furiosus
330
-
 substrate: genistin, pH 6.0, 95°C Pyrococcus furiosus
912
-
 substrate: 4-nitrophenyl-beta-D-glucopyranoside, pH 6.0, 95°C Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-glucopyranose
-
 ?
daidzin + H2O
-
 Pyrococcus furiosus beta-D-glucose + daidzein
-
 ?
genistin + H2O
-
 Pyrococcus furiosus beta-D-glucose + genistein
-
 ?
glycitin + H2O i.e. glycitein 7-O-beta-glucoside Pyrococcus furiosus beta-D-glucose + glycitein
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 56000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
75 98 75°C: about 50% of maximal activity, 98°C: about 85% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 half-life: 1020 h Pyrococcus furiosus
75
-
 half-life: 476 h Pyrococcus furiosus
80
-
 half-life: 307 h Pyrococcus furiosus
85
-
 half-life: 181 h Pyrococcus furiosus
90
-
 half-life: 96 h Pyrococcus furiosus
95
-
 half-life: 59 h Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
610
-
 glycitin pH 6.0, 95°C Pyrococcus furiosus
1880
-
 daidzin pH 6.0, 95°C Pyrococcus furiosus
6050
-
 genistin pH 6.0, 95°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: 80% of maximal activity, pH 8.0: about 80% of maximal activity Pyrococcus furiosus